BBa_K844000 1 BBa_K844000 10x-Histidine (10x-His) Tag with double stop codon (TAATAA) 2012-10-01T11:00:00Z 2015-05-08T01:13:33Z Constructed through oligonucleotide annealing Released HQ 2013 10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part. false false _1104_ 0 9404 9 In stock true none false Kathleen Miller annotation2206607 1 10x-Histidine Tag range2206607 1 1 30 annotation2206609 1 Stop range2206609 1 34 36 annotation2206608 1 Stop range2206608 1 31 33 BBa_J34803 1 BBa_J34803 ribosome binding site 2006-10-25T11:00:00Z 2015-08-31T04:08:46Z a Released HQ 2013 a false false _62_ 0 1085 62 In stock false a false Alexandra Choutko BBa_K1988001 1 BBa_K1988001 his-tagged ppx2 homologue w/ Anderson Promoter J23106 2016-10-13T11:00:00Z 2016-10-14T09:44:11Z Genomic sequence from paper 'Deciphering the Genome of Polyphosphate Accumulating Actino bacterium Microlunatus Phosphovorus' by Kawakoshi, et. al. Name: C- Terminus His-tagged Exopolyphosphatase with Anderson promoter J23106 Locus tag: MLP_44770 Function: There are two types of exopolyphosphatase, PPX1 and PPX2, both of which mediate the hydrolysis of the terminal phosphate of a polyphosphate chain. [1] In Microlunatus phosphovorus:</b> While most Actinobacteria species have both PPX1 and PPX2, M. phosphovorus only has one PPX2 homolog. [1] Cofactors and Modifications: </b> There are currently no known cofactors and modifications of the PPX2 homolog in M. phosphovorus. 3-D Structure: C-score: 1.01 The three-dimensional structure was predicted using the I-TASSER software [3]. All images shown are the models with the highest confidence, which is quantitatively measured with a C-score that typically falls within the range [-5,2], in which 2 represents the highest level of confidence, and -5 represents the lowest level of confidence. Gene length: 945 base pairs [4] Protein size:33.0 kDa [4] pH Range The PPX2 homolog in M. phosphovorus has yet to be characterized, and so there is no data for the pH range in which it functions. Temperature Range The PPX2 homolog in M. phosphovorus has yet to be characterized, and so there is no data for the temperature range in which it functions. References [1] A. Kawakoshi, H. Nakazawa, J. Fukada, M. Sasagawa, Y. Katano, S. Nakamura, A. Hosoyama, H. Sasaki, N. Ichikawa, S. Hanada, Y. Kamagata, K. Nakamura, S. Yamazaki and N. Fujita, "Deciphering the Genome of Polyphosphate Accumulating Actinobacterium Microlunatus phosphovorus", DNA Research, vol. 19, no. 5, pp. 383-394, 2012. [2] [3] Yang, R. Yan, A. Roy, D. Xu, J. Poisson and Y. Zhang, "The I-TASSER Suite: protein structure and function prediction", Nature Methods, vol. 12, no. 1, pp. 7-8, 2014. [4] "ppx - Exopolyphosphatase - Microlunatus phosphovorus (strain ATCC 700054 / DSM 10555 / JCM 9379 / NBRC 101784 / NCIMB 13414 / VKM Ac-1990 / NM-1) - ppgK gene & protein", Uniprot, 2016. [Online]. Available: http://www.uniprot.org/uniprot/F5XI06. [Accessed: 22- Jul- 2016]. false false _2455_ 26037 26037 9 false Genomic sequence optimized for E. Coli K-12 using IDT Codon optimizer. false Bowman Clark component2506616 1 BBa_K1988000 component2506620 1 BBa_K844000 component2506615 1 BBa_J34803 component2506614 1 BBa_J23106 annotation2506615 1 BBa_J34803 range2506615 1 44 56 annotation2506620 1 BBa_K844000 range2506620 1 1015 1050 annotation2506616 1 BBa_K1988000 range2506616 1 65 1006 annotation2506614 1 BBa_J23106 range2506614 1 1 35 BBa_J23106 1 BBa_J23106 constitutive promoter family member 2006-08-13T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K1988000 1 BBa_K1988000 ppx2 homologue: exopolyphosphatase from M. phosphovorus 2016-10-13T11:00:00Z 2016-10-14T08:24:33Z This part sequence was obtained from 'Deciphering the Genome of Polyphosphate Accumulating Actinobacterium Microlunatus Phosphovorus' by Kawakoshi, et. al. <h1>PPX2 Homolog</h1> <p><b>Name:</b>Exopolyphosphatase</p> <p><b>Locus tag: </b> MLP_44770</p> <p><b>Function: </b> </p> There are two types of exopolyphosphatase, PPX1 and PPX2, both of which mediate the hydrolysis of the terminal phosphate of a polyphosphate chain. [1] In <i>Microlunatus phosphovorus</i>:</b> While most Actinobacteria species have both PPX1 and PPX2, <i>M. phosphovorus</i> only has one PPX2 homolog. [1] Cofactors and Modifications: </b> There are currently no known cofactors and modifications of the PPX2 homolog in M. phosphovorus. 3-D Structure: C-score: 1.01 The three-dimensional structure was predicted using the I-TASSER software [3]. All images shown are the models with the highest confidence, which is quantitatively measured with a C-score that typically falls within the range [-5,2], in which 2 represents the highest level of confidence, and -5 represents the lowest level of confidence. Gene length: 945 base pairs [4] Protein size:33.0 kDa [4] pH Range The PPX2 homolog in M. phosphovorus has yet to be characterized, and so there is no data for the pH range in which it functions. Temperature Range The PPX2 homolog in M. phosphovorus has yet to be characterized, and so there is no data for the temperature range in which it functions. <h2>References</h2> [1] A. Kawakoshi, H. Nakazawa, J. Fukada, M. Sasagawa, Y. Katano, S. Nakamura, A. Hosoyama, H. Sasaki, N. Ichikawa, S. Hanada, Y. Kamagata, K. Nakamura, S. Yamazaki and N. Fujita, "Deciphering the Genome of Polyphosphate Accumulating Actinobacterium <i>Microlunatus phosphovorus</i>", <i>DNA Research</i>, vol. 19, no. 5, pp. 383-394, 2012.<br> [2] <br> [3] Yang, R. Yan, A. Roy, D. Xu, J. Poisson and Y. Zhang, "The I-TASSER Suite: protein structure and function prediction", <i>Nature Methods</i>, vol. 12, no. 1, pp. 7-8, 2014. <br> [4] "ppx - Exopolyphosphatase - <i>Microlunatus phosphovorus</i> (strain ATCC 700054 / DSM 10555 / JCM 9379 / NBRC 101784 / NCIMB 13414 / VKM Ac-1990 / NM-1) - ppgK gene & protein", <i>Uniprot</i>, 2016. [Online]. Available: http://www.uniprot.org/uniprot/F5XI06. [Accessed: 22- Jul- 2016]. false false _2455_ 26037 26037 9 false Gene was optimized for E. coli K-12 using IDT codon optimization false Bowman Clark BBa_K1988000_sequence 1 gtggccacggaaccagttcgcgttggagctatcgactgcggaacaaatagtatccgtttgttaatcgcagaggcacgcgttgatggcagcttaattgaccttgtacgcgagttggagatcgtacgccttggtcaaggagtggacgccactggggagttacacccagacgctcttgctcgcacctttgcggcagccgaacgttatggggaacagctgcgcgcttacggcgtgcgccccgagcgcacgcgcgttgttgctacatcggccacccgtgacgctcgtaatgtcgaggatttcgatgcgggcatggctgcacgtttaggagtacgcccagagattatcagcggaacagaggaagcacgcttatctttcacaggcgcgttgtcgggcgttcgtgtggcctccgatggtcctatcttggttatggacatcggaggagggagcactgaacttgtgctgggtgatcgtacgggtcgtatcgatcgtgccgtcagcttgaatatcgggtccgtgcgtctgactgaacgcttcgggctggtgggcccgctgaccacggaagcacgtcagagcgctgcggcctatgtggacggccttcttgaccaattagaatggccctcgggcttactggggcaaattggctcttggattggagtggccggcacagtaacgacaatgagcgcccttaaacaaggccttatttcgtatgaccgcagtaaagtgcatggtagtgttctgtcacttgcagagatcgcggggttgtctaatcgcctggcgacgcttacaacggaggagatccacgatcttggagtcccggcggggcgcgctgacgttatcactgctggctctcttatcgccgatcgcgttgccaaccgtgtgggccgcccgatgattgtgagtgaaagcgacattttagacggcattgtgcttggcttgctggcacgt BBa_J34803_sequence 1 tcacacaggaaag BBa_K1988001_sequence 1 tttacggctagctcagtcctaggtatagtgctagctactagagtcacacaggaaagtactagaggtggccacggaaccagttcgcgttggagctatcgactgcggaacaaatagtatccgtttgttaatcgcagaggcacgcgttgatggcagcttaattgaccttgtacgcgagttggagatcgtacgccttggtcaaggagtggacgccactggggagttacacccagacgctcttgctcgcacctttgcggcagccgaacgttatggggaacagctgcgcgcttacggcgtgcgccccgagcgcacgcgcgttgttgctacatcggccacccgtgacgctcgtaatgtcgaggatttcgatgcgggcatggctgcacgtttaggagtacgcccagagattatcagcggaacagaggaagcacgcttatctttcacaggcgcgttgtcgggcgttcgtgtggcctccgatggtcctatcttggttatggacatcggaggagggagcactgaacttgtgctgggtgatcgtacgggtcgtatcgatcgtgccgtcagcttgaatatcgggtccgtgcgtctgactgaacgcttcgggctggtgggcccgctgaccacggaagcacgtcagagcgctgcggcctatgtggacggccttcttgaccaattagaatggccctcgggcttactggggcaaattggctcttggattggagtggccggcacagtaacgacaatgagcgcccttaaacaaggccttatttcgtatgaccgcagtaaagtgcatggtagtgttctgtcacttgcagagatcgcggggttgtctaatcgcctggcgacgcttacaacggaggagatccacgatcttggagtcccggcggggcgcgctgacgttatcactgctggctctcttatcgccgatcgcgttgccaaccgtgtgggccgcccgatgattgtgagtgaaagcgacattttagacggcattgtgcttggcttgctggcacgttactagagcatcatcaccatcaccaccatcatcaccattaataa BBa_J23106_sequence 1 tttacggctagctcagtcctaggtatagtgctagc BBa_K844000_sequence 1 catcatcaccatcaccaccatcatcaccattaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z