BBa_K1989005
1
BBa_K1989005
Quadruple spytag with SUP and His-tag
2016-10-12T11:00:00Z
2016-10-14T02:43:59Z
Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant. SUP is a completely artificial protein from structure calculating to function modifying.
In the last few years, hydrogens made from natural or synthetic polymers have been investigated due to their extensive application in clinical medicine and synthetic biology. Compared to traditional biological material, protein-based multifunctional biological material is low-cost, facile and eco-friendly. However, strategies for assembling 3D molecular networks synthesized only by protein molecular remain underdeveloped. The reason why investigating this technology is still tough is lack of protein-based cross linking agents. Inspired by the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtained two peptide called SpyTag(the short one) and SpyCatcher(the long one) which are able to form isopeptide bond with the other without any assistant. By fusing SpyTag and SpyCatcher with functional domains respectively, researchers solved the problem tactfully. In order to using SpyTag and SpyCatcher system as scaffold, we fused three SpyTag spaced by (VPGVG)4 with 6xHistag in N-terminal and another functional protein called Super Uranyl-binding Protein(SUP) in C-terminal.
false
false
_2456_
32647
17187
9
false
The length of the linker between spytag and SUP may affect the physical and chemical property of hydrogel.
false
Yiming Dong
annotation2512454
1
Quadruple spytag
range2512454
1
69
443
annotation2512453
1
his-tag
range2512453
1
30
68
annotation2512455
1
SUP
range2512455
1
528
764
BBa_K1989005_sequence
1
atggtttaactttaagaaggagatataccatgaaaggttcttctcatcatcatcatcatcacgttgacgcccatattgttatggtggatgcatataaaccgaccaaactggatggtcatggtgtgggtgttccgggtgttggtgtgcctggcgttggcgttcctggtgtgggcgaactggcacatatcgtgatggttgatgcctacaaacctacaaaaaccagcgtgccaggtgttggagtaccgggtgtaggcgttccaggtgaaggtggtctgctggatgcgcacattgtaatggttgacgcgtataaaccaacgaaaaccagcgttccgggtgttggtgttcctggtgaaggtgtgcctggtgtgggtgtaccaggcgtaggtgttccaggtggtgaactggcacatatcgtgatggttgatgcctacaaacctacaaaaaccagcgttccgggtgttggtgttcctggtgaaggtgtgcctggtgtgggtgtaccaggcgtaggtgttccaggtggtgaactgctggattgccgtgaacgcattgaaaaagacctggaaaacctggaaaaagaactgatggaaatgaaaagcatcaaactgtctgatgacgaagaagcggtggttgaacgtgccctgaattatcgcgatgacagtgtctattacctggaaaaaggcgatcatattacctcctttggttgtatcacgtacgcgcagggcctgctggatagcctgcgtatgctgcaccgcattatcgaaggttaacaaagcccgaaaggaagctgagttggcttaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z