BBa_K1989009 1 BBa_K1989009 Triple spytag with SUP, His-tag, FlAsH-tag and signal peptide PhoA 2016-10-07T11:00:00Z 2016-10-08T10:33:07Z Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant. SUP is a completely artificial protein from structure calculating to function modifying. The fused protein PhoA-Histag-3A-SUP-FlAsHtag possess both isopeptide bond forming function, uranyl-binding ability and secretion . Thus, using PhoA-Histag-3A-SUP-FlAsHtag as a part of hydrogel formation, we can obtain our multifunctional biomaterial. false false _2456_ 17187 17187 9 false The length of the linker between spytag and SUP may affect the physical and chemical property of hydrogel. As several attributes of our target proteins, especially their folding state in cells, we were unable to rationally design or choose a signal peptide for each. Nevertheless, we could build a Signal Peptide Repertory to screen for the best SP candidate. Our ultimate goal was to select the most appropriate ones from a huge amount of signal peptides. false Yiming Dong annotation2488639 1 FlAsH-tag range2488639 1 702 744 annotation2488638 1 SUP range2488638 1 466 701 annotation2488633 1 His-tag range2488633 1 91 129 annotation2488628 1 Spytag-Spytag-Spytag range2488628 1 130 377 annotation2488632 1 Signal peptide PhoA range2488632 1 24 90 annotation2488636 1 Linker range2488636 1 382 461 BBa_K1989009_sequence 1 tggtttaactttaagaaggagatatacatgaaacagtctaccatcgctctggctctgctgccgctgctgttcaccccggttaccaaagctatgaaaggttcttctcatcatcatcatcatcacgttgacgcccatattgttatggtggatgcatataaaccgaccaaactggatggtcatggtgtgggtgttccgggtgttggtgtgcctggcgttggcgttcctggtgtgggcgaactggcacatatcgtgatggttgatgcctacaaacctacaaaaaccagcgtgccaggtgttggagtaccgggtgtaggcgttccaggtgaaggtggtctgctggatgcgcacattgtaatggttgacgcgtataaaccaacgaaaaccagcgttccgggtgttggtgttcctggtgaaggtgtgcctggtgtgggtgtaccaggcgtaggtgttccaggtggtgaactgctggattgccgtgaacgcattgaaaaagacctggaagacctggaaaaagaactgatggaaatgaaaagcatcaaactgtctgatgacgaagaagcggtggttgaacgtgccctgaattatcgcgatgacagtgtctattacctggaaaaaggcgatcatattacctcctttggttgtatcacgtacgcggagggcctgacggatagcctgcgtatgctgcaccgcattatcgaaggttccttcctgaactgctgcccgggttgctgcatggaaccgtaacaaagcccgaaaggaagctgagttggcttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z