BBa_K1989011 1 BBa_K1989011 Triple spytag with SUP, His-tag , flash tag and signal peptide PelB 2016-10-08T11:00:00Z 2016-10-09T12:34:28Z Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant. SUP is a completely artificial protein from structure calculating to function modifying. The fused protein PelB-Histag-3A-SUP-FlAsHtag possess both isopeptide bond forming function, uranyl-binding ability and secretion . Thus, using PelB-Histag-3A-SUP-FlAsHtag as a part of hydrogel formation, we can obtain our multifunctional biomaterial. false false _2456_ 17187 17187 9 false The length of the linker between spytag and SUP may affect the physical and chemical property of hydrogel. As several attributes of our target proteins, especially their folding state in cells, we were unable to rationally design or choose a signal peptide for each. Nevertheless, we could build a Signal Peptide Repertory to screen for the best SP candidate. Our ultimate goal was to select the most appropriate ones from a huge amount of signal peptides. false Yiming Dong annotation2488692 1 FlAsH-tag range2488692 1 701 743 annotation2488674 1 Signal peptide PelB range2488674 1 24 89 annotation2488689 1 Spytag-Spytag-Spytag range2488689 1 129 376 annotation2488688 1 His-tag range2488688 1 90 128 annotation2488690 1 Linker range2488690 1 381 460 annotation2488691 1 SUP range2488691 1 465 700 BBa_K1989011_sequence 1 tggtttaactttaagaaggagatatgaaatacctgctgccgaccgctgctgctggtctgctgctgctggctgctcagccggctatggctatgaaaggttcttctcatcatcatcatcatcacgttgacgcccatattgttatggtggatgcatataaaccgaccaaactggatggtcatggtgtgggtgttccgggtgttggtgtgcctggcgttggcgttcctggtgtgggcgaactggcacatatcgtgatggttgatgcctacaaacctacaaaaaccagcgtgccaggtgttggagtaccgggtgtaggcgttccaggtgaaggtggtctgctggatgcgcacattgtaatggttgacgcgtataaaccaacgaaaaccagcgttccgggtgttggtgttcctggtgaaggtgtgcctggtgtgggtgtaccaggcgtaggtgttccaggtggtgaactgctggattgccgtgaacgcattgaaaaagacctggaagacctggaaaaagaactgatggaaatgaaaagcatcaaactgtctgatgacgaagaagcggtggttgaacgtgccctgaattatcgcgatgacagtgtctattacctggaaaaaggcgatcatattacctcctttggttgtatcacgtacgcggagggcctgacggatagcctgcgtatgctgcaccgcattatcgaaggttccttcctgaactgctgcccgggttgctgcatggaaccgtaacaaagcccgaaaggaagctgagttggcttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z