BBa_K1989012 1 BBa_K1989012 Triple spytag with SUP, FlAsH-tag and signal peptide ImdA 2016-10-08T11:00:00Z 2016-10-09T03:33:52Z Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant. SUP is a completely artificial protein from structure calculating to function modifying. The fused protein ImdA-Histag-3A-SUP-FlAsHtag possess both isopeptide bond forming function, uranyl-binding ability and secretion . Thus, using ImdA-Histag-3A-SUP-FlAsHtag as a part of hydrogel formation, we can obtain our multifunctional biomaterial. false false _2456_ 17187 17187 9 false The length of the linker between spytag and SUP may affect the physical and chemical property of hydrogel. As several attributes of our target proteins, especially their folding state in cells, we were unable to rationally design or choose a signal peptide for each. Nevertheless, we could build a Signal Peptide Repertory to screen for the best SP candidate. Our ultimate goal was to select the most appropriate ones from a huge amount of signal peptides. false Yiming Dong annotation2488709 1 FlAsH-tag range2488709 1 767 805 annotation2488706 1 RBS range2488706 1 30 61 annotation2488707 1 Signal peptide ImdA range2488707 1 62 151 annotation2488708 1 Spytag-Spytag-Spytag-SUP range2488708 1 155 762 BBa_K1989012_sequence 1 tgaacttaagcaaaaggagagggacgcgtgcgcaaccaacactcgctagaccaggtcctacatgatgaacctttctcgtcgtacacttcttacaacaggctctgctgctacacttgcttacgctcttggcatggctggctctgctcaagctaacgcccacattgtcatggtcgacgcgtataaaccgaccaaactggacggtcacggcgttggcgttccgggtgtaggcgttccgggcgttggtgttccgggcgtaggcgaactggctcatattgttatggtcgacgcgtacaaaccgaccaaaacctctgttccgggcgttggcgttccgggtgtaggcgttccgggcgaaggcggtctgctggatgcccatattgtcatggtcgacgcgtataaaccgacgaaaacctctgttccgggcgttggtgttccgggcgaaggcgttccgggcgtaggtgttccgggcgttggtgttccgggcggtgaactgctggattgtcgcgaacgtatcgagaaagacctggagaacctggagaaagagctgatggagatgaaaagcatcaaactgtcggacgatgaagaagcggttgttgaacgcgcactgaactatcgcgacgatagcgtctactacctggaaaaaggcgatcacatcaccagcttcggttgtatcacctacgcacaaggtctgctggattctctgcgtatgctgcatcgcatcatcgaaggtatgaaaggcagcagccaccatcatcatcatcacgttgatttccttaactgctgccctggctgctgcatggaaccttaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z