BBa_K1989013 1 BBa_K1989013 Triple spytag with SUP, FlAsH-tag and signal peptide NprE 2016-10-08T11:00:00Z 2016-10-09T03:34:52Z Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant. SUP is a completely artificial protein from structure calculating to function modifying. The fused protein NprE-Histag-3A-SUP-FlAsHtag possess both isopeptide bond forming function, uranyl-binding ability and secretion . Thus, using NprE-Histag-3A-SUP-FlAsHtag as a part of hydrogel formation, we can obtain our multifunctional biomaterial. false false _2456_ 17187 17187 9 false The length of the linker between spytag and SUP may affect the physical and chemical property of hydrogel. As several attributes of our target proteins, especially their folding state in cells, we were unable to rationally design or choose a signal peptide for each. Nevertheless, we could build a Signal Peptide Repertory to screen for the best SP candidate. Our ultimate goal was to select the most appropriate ones from a huge amount of signal peptides. false Yiming Dong annotation2488713 1 FlAsH-tag range2488713 1 756 794 annotation2488711 1 Signal peptide NprE range2488711 1 60 140 annotation2488712 1 Spytag-Spytag-Spytag-SUP range2488712 1 144 751 annotation2488710 1 RBS range2488710 1 30 59 BBa_K1989013_sequence 1 tgaacttaagcaaaaggagagggacgcgtcacaaaagaaaagaaggaggagcgaaatgaatgggccttggcaaaaaactttctgttgccgttgctgcaagcttcatgtctctttcgatctctcttcctggcgttcaagctaacgcccacattgtcatggtcgacgcgtataaaccgaccaaactggacggtcacggcgttggcgttccgggtgtaggcgttccgggcgttggtgttccgggcgtaggcgaactggctcatattgttatggtcgacgcgtacaaaccgaccaaaacctctgttccgggcgttggcgttccgggtgtaggcgttccgggcgaaggcggtctgctggatgcccatattgtcatggtcgacgcgtataaaccgacgaaaacctctgttccgggcgttggtgttccgggcgaaggcgttccgggcgtaggtgttccgggcgttggtgttccgggcggtgaactgctggattgtcgcgaacgtatcgagaaagacctggagaacctggagaaagagctgatggagatgaaaagcatcaaactgtcggacgatgaagaagcggttgttgaacgcgcactgaactatcgcgacgatagcgtctactacctggaaaaaggcgatcacatcaccagcttcggttgtatcacctacgcacaaggtctgctggattctctgcgtatgctgcatcgcatcatcgaaggtatgaaaggcagcagccaccatcatcatcatcacgttgatttccttaactgctgccctggctgctgcatggaaccttaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z