BBa_K1989013
1
BBa_K1989013
Triple spytag with SUP, FlAsH-tag and signal peptide NprE
2016-10-08T11:00:00Z
2016-10-09T03:34:52Z
Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant. SUP is a completely artificial protein from structure calculating to function modifying.
The fused protein NprE-Histag-3A-SUP-FlAsHtag possess both isopeptide bond forming function, uranyl-binding ability and secretion . Thus, using NprE-Histag-3A-SUP-FlAsHtag as a part of hydrogel formation, we can obtain our multifunctional biomaterial.
false
false
_2456_
17187
17187
9
false
The length of the linker between spytag and SUP may affect the physical and chemical property of hydrogel. As several attributes of our target proteins, especially their folding state in cells, we were unable to rationally design or choose a signal peptide for each. Nevertheless, we could build a Signal Peptide Repertory to screen for the best SP candidate. Our ultimate goal was to select the most appropriate ones from a huge amount of signal peptides.
false
Yiming Dong
annotation2488713
1
FlAsH-tag
range2488713
1
756
794
annotation2488711
1
Signal peptide NprE
range2488711
1
60
140
annotation2488712
1
Spytag-Spytag-Spytag-SUP
range2488712
1
144
751
annotation2488710
1
RBS
range2488710
1
30
59
BBa_K1989013_sequence
1
tgaacttaagcaaaaggagagggacgcgtcacaaaagaaaagaaggaggagcgaaatgaatgggccttggcaaaaaactttctgttgccgttgctgcaagcttcatgtctctttcgatctctcttcctggcgttcaagctaacgcccacattgtcatggtcgacgcgtataaaccgaccaaactggacggtcacggcgttggcgttccgggtgtaggcgttccgggcgttggtgttccgggcgtaggcgaactggctcatattgttatggtcgacgcgtacaaaccgaccaaaacctctgttccgggcgttggcgttccgggtgtaggcgttccgggcgaaggcggtctgctggatgcccatattgtcatggtcgacgcgtataaaccgacgaaaacctctgttccgggcgttggtgttccgggcgaaggcgttccgggcgtaggtgttccgggcgttggtgttccgggcggtgaactgctggattgtcgcgaacgtatcgagaaagacctggagaacctggagaaagagctgatggagatgaaaagcatcaaactgtcggacgatgaagaagcggttgttgaacgcgcactgaactatcgcgacgatagcgtctactacctggaaaaaggcgatcacatcaccagcttcggttgtatcacctacgcacaaggtctgctggattctctgcgtatgctgcatcgcatcatcgaaggtatgaaaggcagcagccaccatcatcatcatcacgttgatttccttaactgctgccctggctgctgcatggaaccttaat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z