BBa_K1989016 1 BBa_K1989016 Triple spytag with SUP, FlAsH-tag and signal peptide LipA 2016-10-08T11:00:00Z 2016-10-09T03:39:29Z Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant. SUP is a completely artificial protein from structure calculating to function modifying. The fused protein LipA-Histag-3A-SUP-FlAsHtag possess both isopeptide bond forming function, uranyl-binding ability and secretion. Thus, using LipA-Histag-3A-SUP-FlAsHtag as a part of hydrogel formation, we can obtain our multifunctional biomaterial. false false _2456_ 17187 17187 9 false The length of the linker between spytag and SUP may affect the physical and chemical property of hydrogel. As several attributes of our target proteins, especially their folding state in cells, we were unable to rationally design or choose a signal peptide for each. Nevertheless, we could build a Signal Peptide Repertory to screen for the best SP candidate. Our ultimate goal was to select the most appropriate ones from a huge amount of signal peptides. false Yiming Dong annotation2488725 1 Signal peptide LipA range2488725 1 55 147 annotation2488724 1 RBS range2488724 1 30 50 annotation2488726 1 Spytag-Spytag-Spytag-SUP range2488726 1 151 758 annotation2488727 1 FlAsH-tag range2488727 1 763 801 BBa_K1989016_sequence 1 tgaacttaagcaaaaggagagggacgcgtaaaactcggaggtcccattctatgaatgaaattcgttaaacgtcgtatcatcgctcttgttacaatccttatgctttctgttacatctcttttcgctcttcaaccttctgctaaagctaacgcccacattgtcatggtcgacgcgtataaaccgaccaaactggacggtcacggcgttggcgttccgggtgtaggcgttccgggcgttggtgttccgggcgtaggcgaactggctcatattgttatggtcgacgcgtacaaaccgaccaaaacctctgttccgggcgttggcgttccgggtgtaggcgttccgggcgaaggcggtctgctggatgcccatattgtcatggtcgacgcgtataaaccgacgaaaacctctgttccgggcgttggtgttccgggcgaaggcgttccgggcgtaggtgttccgggcgttggtgttccgggcggtgaactgctggattgtcgcgaacgtatcgagaaagacctggagaacctggagaaagagctgatggagatgaaaagcatcaaactgtcggacgatgaagaagcggttgttgaacgcgcactgaactatcgcgacgatagcgtctactacctggaaaaaggcgatcacatcaccagcttcggttgtatcacctacgcacaaggtctgctggattctctgcgtatgctgcatcgcatcatcgaaggtatgaaaggcagcagccaccatcatcatcatcacgttgatttccttaactgctgccctggctgctgcatggaaccttaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z