BBa_K1989017
1
BBa_K1989017
Triplespycatcher with His-tag , FlAsH-tag and signal peptide OmpA
2016-10-08T11:00:00Z
2016-10-09T02:30:57Z
Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant.
The fused protein OmpA-Histag-3B-FlAsHtag possess both isopeptide bond forming function, uranyl-binding ability and secretion. Thus, using OmpA-Histag-3B-FlAsHtag as a part of hydrogel formation, we can obtain our multifunctional biomaterial.
false
false
_2456_
17187
17187
9
false
The length of the linker between spycatcher may affect the physical and chemical property of hydrogel. As several attributes of our target proteins, especially their folding state in cells, we were unable to rationally design or choose a signal peptide for each. Nevertheless, we could build a Signal Peptide Repertory to screen for the best SP candidate. Our ultimate goal was to select the most appropriate ones from a huge amount of signal peptides.
false
Yiming Dong
annotation2488730
1
Signal peptide OmpA
range2488730
1
24
90
annotation2488731
1
His-tag
range2488731
1
91
129
annotation2488738
1
FlAsH-tag
range2488738
1
1760
1802
annotation2488735
1
Spycatcher-Spycatcher-Spycatcher
range2488735
1
133
1740
BBa_K1989017_sequence
1
tggtttaactttaagaaggagatatacatgaaaaaaaccgctatcgctatcgctgttgctctggctggtttcgctaccgttgctcaggctatgaaaggttcttctcatcatcatcatcatcacgttgacgtcgacatcccaacgaccgaaaacctgtattttcagggcgccatggttgataccttatcaggtttatcaagtgagcaaggtcagtccggtgatatgacaattgaagaagatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatattgacggtccgcaaggtatttggggtcagctcgacggccacggcgtgggtgttccgggcgtcggtgtgccgggtgtgggtgtgccgggcgaaggtgtgccgggcgtcggtgtgccgggtgttggtgttccgggcgttggtgtgccgggcgttggcgtgccgggcgagggtgtgccgggcgttggtgttccgggcgtgggtgtgccgggcgtgggcgtgccgggcgtcggtgttccgggcgagggtgtgccgggcgtaggtgtgccgggcgttggtgagctcgatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatattactagatcgtggacgtctagtgtgccgggcgtcggcgtgccgggcgtaggtgttccgggcgagggtgttccgggcgttggtgtgccgggcgtcggcgtgccgggcgtgggtgttccgggcgtaggtgtgccgggcgagggtgtgccgggcgtgggcgtgccgggcgtaggtgttccgggcgtaggtgttccgggcgtaggtgttccgggtgaaggcgtgccgggcgttggtgtgccgggtgtgggcgtgccgggcgggctgctcgacatcccaacgaccgaaaacctgtattttcagggcgccatggttgataccttatcaggtttatcaagtgagcaaggtcagtccggtgatatgacaattgaagaagatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatattgacggtccgcaaggtatttggggtcagctcgagtggaagaaataagttccttcctgaactgctgcccgggttgctgcatggaaccgtaacaaagcccgaaaggaagctgagttggcttaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z