BBa_K1989017 1 BBa_K1989017 Triplespycatcher with His-tag , FlAsH-tag and signal peptide OmpA 2016-10-08T11:00:00Z 2016-10-09T02:30:57Z Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant. The fused protein OmpA-Histag-3B-FlAsHtag possess both isopeptide bond forming function, uranyl-binding ability and secretion. Thus, using OmpA-Histag-3B-FlAsHtag as a part of hydrogel formation, we can obtain our multifunctional biomaterial. false false _2456_ 17187 17187 9 false The length of the linker between spycatcher may affect the physical and chemical property of hydrogel. As several attributes of our target proteins, especially their folding state in cells, we were unable to rationally design or choose a signal peptide for each. Nevertheless, we could build a Signal Peptide Repertory to screen for the best SP candidate. Our ultimate goal was to select the most appropriate ones from a huge amount of signal peptides. false Yiming Dong annotation2488730 1 Signal peptide OmpA range2488730 1 24 90 annotation2488731 1 His-tag range2488731 1 91 129 annotation2488738 1 FlAsH-tag range2488738 1 1760 1802 annotation2488735 1 Spycatcher-Spycatcher-Spycatcher range2488735 1 133 1740 BBa_K1989017_sequence 1 tggtttaactttaagaaggagatatacatgaaaaaaaccgctatcgctatcgctgttgctctggctggtttcgctaccgttgctcaggctatgaaaggttcttctcatcatcatcatcatcacgttgacgtcgacatcccaacgaccgaaaacctgtattttcagggcgccatggttgataccttatcaggtttatcaagtgagcaaggtcagtccggtgatatgacaattgaagaagatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatattgacggtccgcaaggtatttggggtcagctcgacggccacggcgtgggtgttccgggcgtcggtgtgccgggtgtgggtgtgccgggcgaaggtgtgccgggcgtcggtgtgccgggtgttggtgttccgggcgttggtgtgccgggcgttggcgtgccgggcgagggtgtgccgggcgttggtgttccgggcgtgggtgtgccgggcgtgggcgtgccgggcgtcggtgttccgggcgagggtgtgccgggcgtaggtgtgccgggcgttggtgagctcgatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatattactagatcgtggacgtctagtgtgccgggcgtcggcgtgccgggcgtaggtgttccgggcgagggtgttccgggcgttggtgtgccgggcgtcggcgtgccgggcgtgggtgttccgggcgtaggtgtgccgggcgagggtgtgccgggcgtgggcgtgccgggcgtaggtgttccgggcgtaggtgttccgggcgtaggtgttccgggtgaaggcgtgccgggcgttggtgtgccgggtgtgggcgtgccgggcgggctgctcgacatcccaacgaccgaaaacctgtattttcagggcgccatggttgataccttatcaggtttatcaagtgagcaaggtcagtccggtgatatgacaattgaagaagatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatattgacggtccgcaaggtatttggggtcagctcgagtggaagaaataagttccttcctgaactgctgcccgggttgctgcatggaaccgtaacaaagcccgaaaggaagctgagttggcttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z