LOCUS       BBa_K1989018            1833 bp    DNA     linear       26-Nov-2024
DEFINITION  Triplespycatcher with His-tag , FlAsH-tag and signal peptide PhoA
ACCESSION   BBa_K1989018
VERSION     BBa_K1989018.1  
FEATURES             Location/Qualifiers
     CDS             24..90
                     /label=Signal peptide PhoA
     misc_feature    91..129
                     /label=His-tag
     CDS             133..1740
                     /label=Spycatcher-Spycatcher-Spycatcher
     misc_feature    1760..1802
                     /label=FlAsH-tag
ORIGIN
        1 tggtttaact ttaagaagga gatatacatg aaacagtcta ccatcgctct ggctctgctg
       61 ccgctgctgt tcaccccggt taccaaagct atgaaaggtt cttctcatca tcatcatcat
      121 cacgttgacg tcgacatccc aacgaccgaa aacctgtatt ttcagggcgc catggttgat
      181 accttatcag gtttatcaag tgagcaaggt cagtccggtg atatgacaat tgaagaagat
      241 agtgctaccc atattaaatt ctcaaaacgt gatgaggacg gcaaagagtt agctggtgca
      301 actatggagt tgcgtgattc atctggtaaa actattagta catggatttc agatggacaa
      361 gtgaaagatt tctacctgta tccaggaaaa tatacatttg tcgaaaccgc agcaccagac
      421 ggttatgagg tagcaactgc tattaccttt acagttaatg agcaaggtca ggttactgta
      481 aatggcaaag caactaaagg tgacgctcat attgacggtc cgcaaggtat ttggggtcag
      541 ctcgacggcc acggcgtggg tgttccgggc gtcggtgtgc cgggtgtggg tgtgccgggc
      601 gaaggtgtgc cgggcgtcgg tgtgccgggt gttggtgttc cgggcgttgg tgtgccgggc
      661 gttggcgtgc cgggcgaggg tgtgccgggc gttggtgttc cgggcgtggg tgtgccgggc
      721 gtgggcgtgc cgggcgtcgg tgttccgggc gagggtgtgc cgggcgtagg tgtgccgggc
      781 gttggtgagc tcgatagtgc tacccatatt aaattctcaa aacgtgatga ggacggcaaa
      841 gagttagctg gtgcaactat ggagttgcgt gattcatctg gtaaaactat tagtacatgg
      901 atttcagatg gacaagtgaa agatttctac ctgtatccag gaaaatatac atttgtcgaa
      961 accgcagcac cagacggtta tgaggtagca actgctatta cctttacagt taatgagcaa
     1021 ggtcaggtta ctgtaaatgg caaagcaact aaaggtgacg ctcatattac tagatcgtgg
     1081 acgtctagtg tgccgggcgt cggcgtgccg ggcgtaggtg ttccgggcga gggtgttccg
     1141 ggcgttggtg tgccgggcgt cggcgtgccg ggcgtgggtg ttccgggcgt aggtgtgccg
     1201 ggcgagggtg tgccgggcgt gggcgtgccg ggcgtaggtg ttccgggcgt aggtgttccg
     1261 ggcgtaggtg ttccgggtga aggcgtgccg ggcgttggtg tgccgggtgt gggcgtgccg
     1321 ggcgggctgc tcgacatccc aacgaccgaa aacctgtatt ttcagggcgc catggttgat
     1381 accttatcag gtttatcaag tgagcaaggt cagtccggtg atatgacaat tgaagaagat
     1441 agtgctaccc atattaaatt ctcaaaacgt gatgaggacg gcaaagagtt agctggtgca
     1501 actatggagt tgcgtgattc atctggtaaa actattagta catggatttc agatggacaa
     1561 gtgaaagatt tctacctgta tccaggaaaa tatacatttg tcgaaaccgc agcaccagac
     1621 ggttatgagg tagcaactgc tattaccttt acagttaatg agcaaggtca ggttactgta
     1681 aatggcaaag caactaaagg tgacgctcat attgacggtc cgcaaggtat ttggggtcag
     1741 ctcgagtgga agaaataagt tccttcctga actgctgccc gggttgctgc atggaaccgt
     1801 aacaaagccc gaaaggaagc tgagttggct taa
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