BBa_K1989018 1 BBa_K1989018 Triplespycatcher with His-tag , FlAsH-tag and signal peptide PhoA 2016-10-08T11:00:00Z 2016-10-09T02:51:28Z Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant. The fused protein PhoA-Histag-3B-FlAsHtag possess both isopeptide bond forming function, uranyl-binding ability and secretion. Thus, using PhoA-Histag-3B-FlAsHtag as a part of hydrogel formation, we can obtain our multifunctional biomaterial. false false _2456_ 17187 17187 9 false The length of the linker between spycatcher may affect the physical and chemical property of hydrogel. As several attributes of our target proteins, especially their folding state in cells, we were unable to rationally design or choose a signal peptide for each. Nevertheless, we could build a Signal Peptide Repertory to screen for the best SP candidate. Our ultimate goal was to select the most appropriate ones from a huge amount of signal peptides. false Yiming Dong annotation2488746 1 Signal peptide PhoA range2488746 1 24 90 annotation2488748 1 His-tag range2488748 1 91 129 annotation2488749 1 Spycatcher-Spycatcher-Spycatcher range2488749 1 133 1740 annotation2488752 1 FlAsH-tag range2488752 1 1760 1802 BBa_K1989018_sequence 1 tggtttaactttaagaaggagatatacatgaaacagtctaccatcgctctggctctgctgccgctgctgttcaccccggttaccaaagctatgaaaggttcttctcatcatcatcatcatcacgttgacgtcgacatcccaacgaccgaaaacctgtattttcagggcgccatggttgataccttatcaggtttatcaagtgagcaaggtcagtccggtgatatgacaattgaagaagatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatattgacggtccgcaaggtatttggggtcagctcgacggccacggcgtgggtgttccgggcgtcggtgtgccgggtgtgggtgtgccgggcgaaggtgtgccgggcgtcggtgtgccgggtgttggtgttccgggcgttggtgtgccgggcgttggcgtgccgggcgagggtgtgccgggcgttggtgttccgggcgtgggtgtgccgggcgtgggcgtgccgggcgtcggtgttccgggcgagggtgtgccgggcgtaggtgtgccgggcgttggtgagctcgatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatattactagatcgtggacgtctagtgtgccgggcgtcggcgtgccgggcgtaggtgttccgggcgagggtgttccgggcgttggtgtgccgggcgtcggcgtgccgggcgtgggtgttccgggcgtaggtgtgccgggcgagggtgtgccgggcgtgggcgtgccgggcgtaggtgttccgggcgtaggtgttccgggcgtaggtgttccgggtgaaggcgtgccgggcgttggtgtgccgggtgtgggcgtgccgggcgggctgctcgacatcccaacgaccgaaaacctgtattttcagggcgccatggttgataccttatcaggtttatcaagtgagcaaggtcagtccggtgatatgacaattgaagaagatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatattgacggtccgcaaggtatttggggtcagctcgagtggaagaaataagttccttcctgaactgctgcccgggttgctgcatggaaccgtaacaaagcccgaaaggaagctgagttggcttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z