LOCUS BBa_K1989019 1823 bp DNA linear 23-Dec-2024 DEFINITION Triplespycatcher with His-tag , FlAsH-tag and signal peptide LTIIB ACCESSION BBa_K1989019 VERSION BBa_K1989019.1 FEATURES Location/Qualifiers CDS 24..80 /label=Signal peptide LTIIb misc_feature 81..119 /label=His-tag CDS 123..1730 /label=Spycatcher-Spycatcher-Spycatcher misc_feature 1750..1792 /label=FlAsH-tag ORIGIN 1 tggtttaact ttaagaagga gattctttca aaaaaatcat caaagctttc gttatcatgg 61 ctgctctggt ttctgttcag atgaaaggtt cttctcatca tcatcatcat cacgttgacg 121 tcgacatccc aacgaccgaa aacctgtatt ttcagggcgc catggttgat accttatcag 181 gtttatcaag tgagcaaggt cagtccggtg atatgacaat tgaagaagat agtgctaccc 241 atattaaatt ctcaaaacgt gatgaggacg gcaaagagtt agctggtgca actatggagt 301 tgcgtgattc atctggtaaa actattagta catggatttc agatggacaa gtgaaagatt 361 tctacctgta tccaggaaaa tatacatttg tcgaaaccgc agcaccagac ggttatgagg 421 tagcaactgc tattaccttt acagttaatg agcaaggtca ggttactgta aatggcaaag 481 caactaaagg tgacgctcat attgacggtc cgcaaggtat ttggggtcag ctcgacggcc 541 acggcgtggg tgttccgggc gtcggtgtgc cgggtgtggg tgtgccgggc gaaggtgtgc 601 cgggcgtcgg tgtgccgggt gttggtgttc cgggcgttgg tgtgccgggc gttggcgtgc 661 cgggcgaggg tgtgccgggc gttggtgttc cgggcgtggg tgtgccgggc gtgggcgtgc 721 cgggcgtcgg tgttccgggc gagggtgtgc cgggcgtagg tgtgccgggc gttggtgagc 781 tcgatagtgc tacccatatt aaattctcaa aacgtgatga ggacggcaaa gagttagctg 841 gtgcaactat ggagttgcgt gattcatctg gtaaaactat tagtacatgg atttcagatg 901 gacaagtgaa agatttctac ctgtatccag gaaaatatac atttgtcgaa accgcagcac 961 cagacggtta tgaggtagca actgctatta cctttacagt taatgagcaa ggtcaggtta 1021 ctgtaaatgg caaagcaact aaaggtgacg ctcatattac tagatcgtgg acgtctagtg 1081 tgccgggcgt cggcgtgccg ggcgtaggtg ttccgggcga gggtgttccg ggcgttggtg 1141 tgccgggcgt cggcgtgccg ggcgtgggtg ttccgggcgt aggtgtgccg ggcgagggtg 1201 tgccgggcgt gggcgtgccg ggcgtaggtg ttccgggcgt aggtgttccg ggcgtaggtg 1261 ttccgggtga aggcgtgccg ggcgttggtg tgccgggtgt gggcgtgccg ggcgggctgc 1321 tcgacatccc aacgaccgaa aacctgtatt ttcagggcgc catggttgat accttatcag 1381 gtttatcaag tgagcaaggt cagtccggtg atatgacaat tgaagaagat agtgctaccc 1441 atattaaatt ctcaaaacgt gatgaggacg gcaaagagtt agctggtgca actatggagt 1501 tgcgtgattc atctggtaaa actattagta catggatttc agatggacaa gtgaaagatt 1561 tctacctgta tccaggaaaa tatacatttg tcgaaaccgc agcaccagac ggttatgagg 1621 tagcaactgc tattaccttt acagttaatg agcaaggtca ggttactgta aatggcaaag 1681 caactaaagg tgacgctcat attgacggtc cgcaaggtat ttggggtcag ctcgagtgga 1741 agaaataagt tccttcctga actgctgccc gggttgctgc atggaaccgt aacaaagccc 1801 gaaaggaagc tgagttggct taa //