BBa_K1989019
1
BBa_K1989019
Triplespycatcher with His-tag , FlAsH-tag and signal peptide LTIIB
2016-10-08T11:00:00Z
2016-10-09T03:00:46Z
Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant.
The fused protein LTIIB-Histag-3B-FlAsHtag possess both isopeptide bond forming function, uranyl-binding ability and secretion. Thus, using LTIIB-Histag-3B-FlAsHtag as a part of hydrogel formation, we can obtain our multifunctional biomaterial.
false
false
_2456_
17187
17187
9
false
The length of the linker between spycatcher may affect the physical and chemical property of hydrogel. As several attributes of our target proteins, especially their folding state in cells, we were unable to rationally design or choose a signal peptide for each. Nevertheless, we could build a Signal Peptide Repertory to screen for the best SP candidate. Our ultimate goal was to select the most appropriate ones from a huge amount of signal peptides.
false
Yiming Dong
annotation2488762
1
Spycatcher-Spycatcher-Spycatcher
range2488762
1
123
1730
annotation2488764
1
FlAsH-tag
range2488764
1
1750
1792
annotation2488761
1
His-tag
range2488761
1
81
119
annotation2488757
1
Signal peptide LTIIb
range2488757
1
24
80
BBa_K1989019_sequence
1
tggtttaactttaagaaggagattctttcaaaaaaatcatcaaagctttcgttatcatggctgctctggtttctgttcagatgaaaggttcttctcatcatcatcatcatcacgttgacgtcgacatcccaacgaccgaaaacctgtattttcagggcgccatggttgataccttatcaggtttatcaagtgagcaaggtcagtccggtgatatgacaattgaagaagatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatattgacggtccgcaaggtatttggggtcagctcgacggccacggcgtgggtgttccgggcgtcggtgtgccgggtgtgggtgtgccgggcgaaggtgtgccgggcgtcggtgtgccgggtgttggtgttccgggcgttggtgtgccgggcgttggcgtgccgggcgagggtgtgccgggcgttggtgttccgggcgtgggtgtgccgggcgtgggcgtgccgggcgtcggtgttccgggcgagggtgtgccgggcgtaggtgtgccgggcgttggtgagctcgatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatattactagatcgtggacgtctagtgtgccgggcgtcggcgtgccgggcgtaggtgttccgggcgagggtgttccgggcgttggtgtgccgggcgtcggcgtgccgggcgtgggtgttccgggcgtaggtgtgccgggcgagggtgtgccgggcgtgggcgtgccgggcgtaggtgttccgggcgtaggtgttccgggcgtaggtgttccgggtgaaggcgtgccgggcgttggtgtgccgggtgtgggcgtgccgggcgggctgctcgacatcccaacgaccgaaaacctgtattttcagggcgccatggttgataccttatcaggtttatcaagtgagcaaggtcagtccggtgatatgacaattgaagaagatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatattgacggtccgcaaggtatttggggtcagctcgagtggaagaaataagttccttcctgaactgctgcccgggttgctgcatggaaccgtaacaaagcccgaaaggaagctgagttggcttaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z