BBa_K1989020 1 BBa_K1989020 Triplespycatcher with His-tag , FlAsH-tag and signal peptide PelB 2016-10-08T11:00:00Z 2016-10-09T03:08:00Z Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant. The fused protein PelB-Histag-3B-FlAsHtag possess both isopeptide bond forming function, uranyl-binding ability and secretion. Thus, using PelB-Histag-3B-FlAsHtag as a part of hydrogel formation, we can obtain our multifunctional biomaterial. false false _2456_ 17187 17187 9 false The length of the linker between spycatcher may affect the physical and chemical property of hydrogel. As several attributes of our target proteins, especially their folding state in cells, we were unable to rationally design or choose a signal peptide for each. Nevertheless, we could build a Signal Peptide Repertory to screen for the best SP candidate. Our ultimate goal was to select the most appropriate ones from a huge amount of signal peptides. false Yiming Dong annotation2488794 1 FlAsH-tag range2488794 1 1759 1801 annotation2488768 1 Signal peptide PelB range2488768 1 24 89 annotation2488786 1 Spycatcher-Spycatcher-Spycatcher range2488786 1 132 1739 annotation2488779 1 His-tag range2488779 1 90 128 BBa_K1989020_sequence 1 tggtttaactttaagaaggagatatgaaatacctgctgccgaccgctgctgctggtctgctgctgctggctgctcagccggctatggctatgaaaggttcttctcatcatcatcatcatcacgttgacgtcgacatcccaacgaccgaaaacctgtattttcagggcgccatggttgataccttatcaggtttatcaagtgagcaaggtcagtccggtgatatgacaattgaagaagatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatattgacggtccgcaaggtatttggggtcagctcgacggccacggcgtgggtgttccgggcgtcggtgtgccgggtgtgggtgtgccgggcgaaggtgtgccgggcgtcggtgtgccgggtgttggtgttccgggcgttggtgtgccgggcgttggcgtgccgggcgagggtgtgccgggcgttggtgttccgggcgtgggtgtgccgggcgtgggcgtgccgggcgtcggtgttccgggcgagggtgtgccgggcgtaggtgtgccgggcgttggtgagctcgatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatattactagatcgtggacgtctagtgtgccgggcgtcggcgtgccgggcgtaggtgttccgggcgagggtgttccgggcgttggtgtgccgggcgtcggcgtgccgggcgtgggtgttccgggcgtaggtgtgccgggcgagggtgtgccgggcgtgggcgtgccgggcgtaggtgttccgggcgtaggtgttccgggcgtaggtgttccgggtgaaggcgtgccgggcgttggtgtgccgggtgtgggcgtgccgggcgggctgctcgacatcccaacgaccgaaaacctgtattttcagggcgccatggttgataccttatcaggtttatcaagtgagcaaggtcagtccggtgatatgacaattgaagaagatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatattgacggtccgcaaggtatttggggtcagctcgagtggaagaaataagttccttcctgaactgctgcccgggttgctgcatggaaccgtaacaaagcccgaaaggaagctgagttggcttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z