BBa_K1989022 1 BBa_K1989022 Triplespycatcher with FlAsH-tag and signal peptide NprE 2016-10-08T11:00:00Z 2016-10-09T04:14:02Z Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant. The fused protein NprE-2B-FlAsHtag possess both isopeptide bond forming function, uranyl-binding ability and secretion. Thus, using NprE-2B-FlAsHtag as a part of hydrogel formation, we can obtain our multifunctional biomaterial. false false _2456_ 17187 17187 9 false The length of the linker between spycatcher may affect the physical and chemical property of hydrogel. As several attributes of our target proteins, especially their folding state in cells, we were unable to rationally design or choose a signal peptide for each. Nevertheless, we could build a Signal Peptide Repertory to screen for the best SP candidate. Our ultimate goal was to select the most appropriate ones from a huge amount of signal peptides. false Yiming Dong annotation2488909 1 Signal peptide NprE range2488909 1 60 140 annotation2488908 1 RBS range2488908 1 30 59 annotation2488910 1 Spycatcher-Spycatcher range2488910 1 144 952 annotation2488912 1 FlAsH-tag range2488912 1 957 995 BBa_K1989022_sequence 1 tgaacttaagcaaaaggagagggacgcgtcacaaaagaaaagaaggaggagcgaaatgaatgggccttggcaaaaaactttctgttgccgttgctgcaagcttcatgtctctttcgatctctcttcctggcgttcaagctaacgccatggttgataccctgtctggtctgagtagcgaacaaggtcaatccggcgatatgaccatcgaagaagacagcgcgacccacatcaaattcagcaaacgcgacgaggacggtaaagaactggctggcgcaaccatggaactgcgcgatagtagcggcaaaaccattagcacctggatcagcgacggtcaggtcaaagatttctacctgtacccgggcaaatacaccttcgttgaaaccgcagcaccggacggttacgaagttgcaaccgcgattaccttcaccgttaacgaacagggtcaggttaccgttaacggcaaagccaccaaaggtgacgcacatattacctctgttccgggtgttggtgttccgggcgaaggcgttccgggtgtaggtgttccgggcgttggcgttccgggtggcgaactggcaatggttgataccctgtctggtctgagtagcgaacaaggtcaatctggtgacatgaccattgaagaagatagcgcgacccacatcaaattcagcaaacgcgacgaggacggtaaagaactggctggcgcaaccatggaactgcgcgatagtagcggtaaaaccattagcacctggattagcgacggccaggttaaagatttctacctgtacccgggcaaatacaccttcgttgaaaccgcagcaccggacggttacgaagttgcgaccgcgattacctttaccgttaacgaacagggccaggttaccgttaacggtaaagcaaccaaaggcgacgcccatatcatgaaaggtagcagccatcaccatcatcatcacgttgatttccttaactgctgccctggctgctgcatggaaccttaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z