BBa_K1989024 1 BBa_K1989024 Triplespycatcher with FlAsH-tag and signal peptide YjfA 2016-10-08T11:00:00Z 2016-10-09T04:32:19Z Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant. The fused protein YjfA-2B-FlAsHtag possess both isopeptide bond forming function, uranyl-binding ability and secretion. Thus, using YjfA-2B-FlAsHtag as a part of hydrogel formation, we can obtain our multifunctional biomaterial. false false _2456_ 17187 17187 9 false The length of the linker between spycatcher may affect the physical and chemical property of hydrogel. As several attributes of our target proteins, especially their folding state in cells, we were unable to rationally design or choose a signal peptide for each. Nevertheless, we could build a Signal Peptide Repertory to screen for the best SP candidate. Our ultimate goal was to select the most appropriate ones from a huge amount of signal peptides. false Yiming Dong annotation2488962 1 FlAsH-tag range2488962 1 957 995 annotation2488961 1 Spycatcher-Spycatcher range2488961 1 144 952 annotation2488960 1 Signal peptide YjfA range2488960 1 57 140 annotation2488959 1 RBS range2488959 1 30 52 BBa_K1989024_sequence 1 tgaacttaagcaaaaggagagggacgcgtgtcatccaccctagggggtaacaatgaatgaaacgtcttttcatgaaagcttctcttgttcttttcgctgttgttttcgttttcgctgttaaaggcgctcctgctaaagctaacgccatggttgataccctgtctggtctgagtagcgaacaaggtcaatccggcgatatgaccatcgaagaagacagcgcgacccacatcaaattcagcaaacgcgacgaggacggtaaagaactggctggcgcaaccatggaactgcgcgatagtagcggcaaaaccattagcacctggatcagcgacggtcaggtcaaagatttctacctgtacccgggcaaatacaccttcgttgaaaccgcagcaccggacggttacgaagttgcaaccgcgattaccttcaccgttaacgaacagggtcaggttaccgttaacggcaaagccaccaaaggtgacgcacatattacctctgttccgggtgttggtgttccgggcgaaggcgttccgggtgtaggtgttccgggcgttggcgttccgggtggcgaactggcaatggttgataccctgtctggtctgagtagcgaacaaggtcaatctggtgacatgaccattgaagaagatagcgcgacccacatcaaattcagcaaacgcgacgaggacggtaaagaactggctggcgcaaccatggaactgcgcgatagtagcggtaaaaccattagcacctggattagcgacggccaggttaaagatttctacctgtacccgggcaaatacaccttcgttgaaaccgcagcaccggacggttacgaagttgcgaccgcgattacctttaccgttaacgaacagggccaggttaccgttaacggtaaagcaaccaaaggcgacgcccatatcatgaaaggtagcagccatcaccatcatcatcacgttgatttccttaactgctgccctggctgctgcatggaaccttaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z