LOCUS BBa_K1989028 901 bp DNA linear 23-Dec-2024 DEFINITION Triple spytag with mSA, His-tag , FlAsH-tag and signal peptide PhoA ACCESSION BBa_K1989028 VERSION BBa_K1989028.1 FEATURES Location/Qualifiers CDS 24..90 /label=Signal peptide PhoA misc_feature 91..129 /label=His-tag CDS 130..377 /label=Spytag-Spytag-Spytag CDS 382..461 /label=Linker CDS 466..824 /label=mSA misc_feature 832..870 /label=FlAsH-tag ORIGIN 1 tggtttaact ttaagaagga gatatacatg aaacagtcta ccatcgctct ggctctgctg 61 ccgctgctgt tcaccccggt taccaaagct atgaaaggtt cttctcatca tcatcatcat 121 cacgttgacg cccatattgt tatggtggat gcatataaac cgaccaaact ggatggtcat 181 ggtgtgggtg ttccgggtgt tggtgtgcct ggcgttggcg ttcctggtgt gggcgaactg 241 gcacatatcg tgatggttga tgcctacaaa cctacaaaaa ccagcgtgcc aggtgttgga 301 gtaccgggtg taggcgttcc aggtgaaggt ggtctgctgg atgcgcacat tgtaatggtt 361 gacgcgtata aaccaacgaa aaccagcgtt ccgggtgttg gtgttcctgg tgaaggtgtg 421 cctggtgtgg gtgtaccagg cgtaggtgtt ccaggtggtg aactggcgga ggcgggtatc 481 accggcacct ggtacaacca gcacggtagc accttcaccg tgaccgcggg tgcggacggt 541 aacctgaccg gtcaatatga aaaccgtgcg cagggtaccg gctgccaaaa cagcccgtac 601 accctgaccg gtcgttataa cggcaccaag ctggagtggc gtgttgaatg gaacaacagc 661 accgagaact gccacagccg taccgaatgg cgtggccagt accaaggtgg cgcggaggcg 721 cgtattaaca cccagtggaa cctgacctat gagggtggca gcggtccggc gaccgaacag 781 ggccaagata cctttaccaa ggtgaaaccg agcgcggcga gcggcagttc cttcctgaac 841 tgctgcccgg gttgctgcat ggaaccgtaa caaagcccga aaggaagctg agttggctta 901 a //