BBa_K1989028
1
BBa_K1989028
Triple spytag with mSA, His-tag , FlAsH-tag and signal peptide PhoA
2016-10-11T11:00:00Z
2016-10-12T09:09:13Z
Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant. By fusing Spytag and Spycatcher with functional domains respectively, researchers solve the problem tactfully. In order to using Spytag and Spycatcher system as scaffold, we fused three Spytag spaced by (VPGVG)4 with 6xHistag in N-terminal and another functional protein called monomeric streptavidin(mSA) in C-terminal.
The fused protein PhoA-Histag-3A-mSA-FlAsHtag possess both isopeptide bond forming function, secretion and collectable. Thus, using PhoA-Histag-3A- mSA -FlAsHtag as a part of hydrogel formation, we can obtain our multifunctional biomaterial.
false
false
_2456_
17187
17187
9
false
The length of the linker between spytag may affect the physical and chemical property of hydrogel. As several attributes of our target proteins, especially their folding state in cells, we were unable to rationally design or choose a signal peptide for each. Nevertheless, we could build a Signal Peptide Repertory to screen for the best SP candidate. Our ultimate goal was to select the most appropriate ones from a huge amount of signal peptides.
false
Yiming Dong
annotation2493907
1
mSA
range2493907
1
466
824
annotation2493910
1
FlAsH-tag
range2493910
1
832
870
annotation2493904
1
His-tag
range2493904
1
91
129
annotation2493905
1
Spytag-Spytag-Spytag
range2493905
1
130
377
annotation2493903
1
Signal peptide PhoA
range2493903
1
24
90
annotation2493906
1
Linker
range2493906
1
382
461
BBa_K1989028_sequence
1
tggtttaactttaagaaggagatatacatgaaacagtctaccatcgctctggctctgctgccgctgctgttcaccccggttaccaaagctatgaaaggttcttctcatcatcatcatcatcacgttgacgcccatattgttatggtggatgcatataaaccgaccaaactggatggtcatggtgtgggtgttccgggtgttggtgtgcctggcgttggcgttcctggtgtgggcgaactggcacatatcgtgatggttgatgcctacaaacctacaaaaaccagcgtgccaggtgttggagtaccgggtgtaggcgttccaggtgaaggtggtctgctggatgcgcacattgtaatggttgacgcgtataaaccaacgaaaaccagcgttccgggtgttggtgttcctggtgaaggtgtgcctggtgtgggtgtaccaggcgtaggtgttccaggtggtgaactggcggaggcgggtatcaccggcacctggtacaaccagcacggtagcaccttcaccgtgaccgcgggtgcggacggtaacctgaccggtcaatatgaaaaccgtgcgcagggtaccggctgccaaaacagcccgtacaccctgaccggtcgttataacggcaccaagctggagtggcgtgttgaatggaacaacagcaccgagaactgccacagccgtaccgaatggcgtggccagtaccaaggtggcgcggaggcgcgtattaacacccagtggaacctgacctatgagggtggcagcggtccggcgaccgaacagggccaagatacctttaccaaggtgaaaccgagcgcggcgagcggcagttccttcctgaactgctgcccgggttgctgcatggaaccgtaacaaagcccgaaaggaagctgagttggcttaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z