BBa_K1989031
1
BBa_K1989031
Triple spytag with mSA, FlAsH-tag and signal peptide ImdA
2016-10-11T11:00:00Z
2016-10-12T11:02:01Z
Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant. By fusing Spytag and Spycatcher with functional domains respectively, researchers solve the problem tactfully. In order to using Spytag and Spycatcher system as scaffold, we fused three Spytag spaced by (VPGVG)4 in N-terminal and another functional protein called monomeric streptavidin(mSA) in C-terminal.
The fused protein ImdA-3A-mSA-FlAsHtag possess both isopeptide bond forming function, secretion and collectable. Thus, using ImdA-3A-mSA-FlAsHtag as a part of hydrogel formation, we can obtain our multifunctional biomaterial.
false
false
_2456_
17187
17187
9
false
The length of the linker between spytag may affect the physical and chemical property of hydrogel. As several attributes of our target proteins, especially their folding state in cells, we were unable to rationally design or choose a signal peptide for each. Nevertheless, we could build a Signal Peptide Repertory to screen for the best SP candidate. Our ultimate goal was to select the most appropriate ones from a huge amount of signal peptides.
false
Yiming Dong
annotation2494258
1
Signal peptide ImdA
range2494258
1
62
151
annotation2494257
1
RBS
range2494257
1
30
61
annotation2494259
1
Spytag-Spytag-Spytag-mSA
range2494259
1
155
850
annotation2494302
1
FlAsH-tag
range2494302
1
855
893
BBa_K1989031_sequence
1
tgaacttaagcaaaaggagagggacgcgtgcgcaaccaacactcgctagaccaggtcctacatgatgaacctttctcgtcgtacacttcttacaacaggctctgctgctacacttgcttacgctcttggcatggctggctctgctcaagctaacgcccacattgtcatggtcgacgcgtataaaccgaccaaactggacggtcacggcgttggcgttccgggtgtaggcgttccgggcgttggtgttccgggcgtaggcgaactggctcatattgttatggtcgacgcgtacaaaccgaccaaaacctctgttccgggcgttggcgttccgggtgtaggcgttccgggcgaaggcggtctgctggatgcccatattgtcatggtcgacgcgtataaaccgacgaaaacctctgttccgggcgttggtgttccgggcgaaggcgttccgggcgtaggtgttccgggcgttggtgttccgggcggtgaactgctggcagaagcaggaatcacaggaacttggtacaaccagcacggaagcacattcacagtcacagcaggagcagacggaaacttaacaggacaatacgaaaaccgggcacaaggaacaggttgtcaaaacagcccgtacacactgacaggaagatacaacggcacgaaacttgaatggcgagtcgaatggaacaacagcacggaaaattgccacagcagaacggaatggagaggacaataccagggaggagcagaagcaagaatcaacacacagtggaacctgacatacgaaggaggatcaggaccggcaacagaacaaggacaagatacgttcacgaaagtaaaaccgagcgcagcaagctgatttccttaactgctgccctggctgctgcatggaaccttaat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z