BBa_K1989032 1 BBa_K1989032 Triple spytag with mSA, FlAsH-tag and signal peptide NprE 2016-10-11T11:00:00Z 2016-10-12T11:37:50Z Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant. By fusing Spytag and Spycatcher with functional domains respectively, researchers solve the problem tactfully. In order to using Spytag and Spycatcher system as scaffold, we fused three Spytag spaced by (VPGVG)4 in N-terminal and another functional protein called monomeric streptavidin(mSA) in C-terminal. The fused protein NprE-3A-mSA-FlAsHtag possess both isopeptide bond forming function, secretion and collectable. Thus, using NprE-3A-mSA-FlAsHtag as a part of hydrogel formation, we can obtain our multifunctional biomaterial. false false _2456_ 17187 17187 9 false The length of the linker between spytag may affect the physical and chemical property of hydrogel. As several attributes of our target proteins, especially their folding state in cells, we were unable to rationally design or choose a signal peptide for each. Nevertheless, we could build a Signal Peptide Repertory to screen for the best SP candidate. Our ultimate goal was to select the most appropriate ones from a huge amount of signal peptides. false Yiming Dong annotation2494384 1 RBS range2494384 1 30 59 annotation2494383 1 Signal peptide NprE range2494383 1 60 140 annotation2494385 1 Spytag-Spytag-Spytag-mSA range2494385 1 144 839 annotation2494386 1 FlAsH-tag range2494386 1 844 882 BBa_K1989032_sequence 1 tgaacttaagcaaaaggagagggacgcgtcacaaaagaaaagaaggaggagcgaaatgaatgggccttggcaaaaaactttctgttgccgttgctgcaagcttcatgtctctttcgatctctcttcctggcgttcaagctaacgcccacattgtcatggtcgacgcgtataaaccgaccaaactggacggtcacggcgttggcgttccgggtgtaggcgttccgggcgttggtgttccgggcgtaggcgaactggctcatattgttatggtcgacgcgtacaaaccgaccaaaacctctgttccgggcgttggcgttccgggtgtaggcgttccgggcgaaggcggtctgctggatgcccatattgtcatggtcgacgcgtataaaccgacgaaaacctctgttccgggcgttggtgttccgggcgaaggcgttccgggcgtaggtgttccgggcgttggtgttccgggcggtgaactgctggcagaagcaggaatcacaggaacttggtacaaccagcacggaagcacattcacagtcacagcaggagcagacggaaacttaacaggacaatacgaaaaccgggcacaaggaacaggttgtcaaaacagcccgtacacactgacaggaagatacaacggcacgaaacttgaatggcgagtcgaatggaacaacagcacggaaaattgccacagcagaacggaatggagaggacaataccagggaggagcagaagcaagaatcaacacacagtggaacctgacatacgaaggaggatcaggaccggcaacagaacaaggacaagatacgttcacgaaagtaaaaccgagcgcagcaagctgatttccttaactgctgccctggctgctgcatggaaccttaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z