BBa_K1989035 1 BBa_K1989035 Triple spytag with mSA, FlAsH-tag and signal peptide LipA 2016-10-11T11:00:00Z 2016-10-12T11:56:57Z Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant. By fusing Spytag and Spycatcher with functional domains respectively, researchers solve the problem tactfully. In order to using Spytag and Spycatcher system as scaffold, we fused three Spytag spaced by (VPGVG)4 in N-terminal and another functional protein called monomeric streptavidin(mSA) in C-terminal. The fused protein LipA-3A-mSA-FlAsHtag possess both isopeptide bond forming function, secretion and collectable. Thus, using LipA-3A-mSA-FlAsHtag as a part of hydrogel formation, we can obtain our multifunctional biomaterial. false false _2456_ 17187 17187 9 false The length of the linker between spytag may affect the physical and chemical property of hydrogel. As several attributes of our target proteins, especially their folding state in cells, we were unable to rationally design or choose a signal peptide for each. Nevertheless, we could build a Signal Peptide Repertory to screen for the best SP candidate. Our ultimate goal was to select the most appropriate ones from a huge amount of signal peptides. false Yiming Dong annotation2494443 1 RBS range2494443 1 30 50 annotation2494444 1 Signal peptide LipA range2494444 1 55 147 annotation2494446 1 FlAsH-tag range2494446 1 851 889 annotation2494445 1 Spytag-Spytag-Spytag-mSA range2494445 1 151 846 BBa_K1989035_sequence 1 tgaacttaagcaaaaggagagggacgcgtaaaactcggaggtcccattctatgaatgaaattcgttaaacgtcgtatcatcgctcttgttacaatccttatgctttctgttacatctcttttcgctcttcaaccttctgctaaagctaacgcccacattgtcatggtcgacgcgtataaaccgaccaaactggacggtcacggcgttggcgttccgggtgtaggcgttccgggcgttggtgttccgggcgtaggcgaactggctcatattgttatggtcgacgcgtacaaaccgaccaaaacctctgttccgggcgttggcgttccgggtgtaggcgttccgggcgaaggcggtctgctggatgcccatattgtcatggtcgacgcgtataaaccgacgaaaacctctgttccgggcgttggtgttccgggcgaaggcgttccgggcgtaggtgttccgggcgttggtgttccgggcggtgaactgctggcagaagcaggaatcacaggaacttggtacaaccagcacggaagcacattcacagtcacagcaggagcagacggaaacttaacaggacaatacgaaaaccgggcacaaggaacaggttgtcaaaacagcccgtacacactgacaggaagatacaacggcacgaaacttgaatggcgagtcgaatggaacaacagcacggaaaattgccacagcagaacggaatggagaggacaataccagggaggagcagaagcaagaatcaacacacagtggaacctgacatacgaaggaggatcaggaccggcaacagaacaaggacaagatacgttcacgaaagtaaaaccgagcgcagcaagctgatttccttaactgctgccctggctgctgcatggaaccttaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z