BBa_K1989040 1 BBa_K1989040 Triplespytag with mRFP, FlAsH-tag and signal peptide ImdA 2016-10-10T11:00:00Z 2016-10-11T10:55:04Z Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant. The fused protein ImdA-3A-mRFP-FlAsHtag possess both isopeptide bond forming function, uranyl-binding ability and secretion. Thus, using ImdA-3A-mRFP-FlAsHtag as a part of hydrogel formation, we can obtain our multifunctional biomaterial. false false _2456_ 17187 17187 9 false The length of the linker between spytag may affect the physical and chemical property of hydrogel. As several attributes of our target proteins, especially their folding state in cells, we were unable to rationally design or choose a signal peptide for each. Nevertheless, we could build a Signal Peptide Repertory to screen for the best SP candidate. Our ultimate goal was to select the most appropriate ones from a huge amount of signal peptides. false Yiming Dong annotation2493143 1 FlAsH-tag range2493143 1 1172 1210 annotation2493141 1 Signal peptide ImdA range2493141 1 62 151 annotation2493142 1 Spytag-Spytag-Spytag-mRFP range2493142 1 155 1168 annotation2493140 1 RBS range2493140 1 30 61 annotation2493156 1 stop range2493156 1 1208 1210 BBa_K1989040_sequence 1 tgaacttaagcaaaaggagagggacgcgtgcgcaaccaacactcgctagaccaggtcctacatgatgaacctttctcgtcgtacacttcttacaacaggctctgctgctacacttgcttacgctcttggcatggctggctctgctcaagctaacgcccacattgtcatggtcgacgcgtataaaccgaccaaactggacggtcacggcgttggcgttccgggtgtaggcgttccgggcgttggtgttccgggcgtaggcgaactggctcatattgttatggtcgacgcgtacaaaccgaccaaaacctctgttccgggcgttggcgttccgggtgtaggcgttccgggcgaaggcggtctgctggatgcccatattgtcatggtcgacgcgtataaaccgacgaaaacctctgttccgggcgttggtgttccgggcgaaggcgttccgggcgtaggtgttccgggcgttggtgttccgggcggtgaactgctgatggcaagcagcgaagacgtcatcaaggaatttatgcggttcaaggtacggatggaaggaagcgtaaacggccacgaatttgaaatcgaaggcgaaggagaaggaagaccttacgaaggaacgcagacagcgaaactgaaagtcacgaagggaggacctttaccgtttgcttgggatatccttagcccgcaattccaatacggaagcaaggcatacgtcaaacatccggcagatattccggactacctgaaacttagcttcccggaaggattcaagtgggaacgggtcatgaacttcgaagacggaggagtagtcacagtcacacaagatagcagccttcaggacggagaatttatttacaaggtaaaactgcggggaacaaacttcccgagcgacggaccggtaatgcagaaaaagacaatgggttgggaagcaagcacagaaaggatgtatccggaagacggagcacttaaaggcgaaatcaagatgcggctgaagctgaaagacggaggacattacgacgcagaagtcaaaacgacgtacatggcgaaaaaaccggtccaacttccgggagcatacaaaacggacatcaagctggacatcacgagccacaacgaagactacacgatcgtcgaacaatacgaacgggcagaaggaagacactctacaggagcagatttccttaactgctgccctggctgctgcatggaaccttaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z