LOCUS BBa_K1989042 1201 bp DNA linear 22-Nov-2024 DEFINITION Triplespytag with mRFP, FlAsH-tag and signal peptide SacB ACCESSION BBa_K1989042 VERSION BBa_K1989042.1 FEATURES Location/Qualifiers RBS 30..50 /label=RBS CDS 55..141 /label=Signal peptide SacB CDS 145..1158 /label=Spytag-Spytag-Spytag-mRFP misc_feature 1162..1200 /label=FlAsH-tag ORIGIN 1 tgaacttaag caaaaggaga gggacgcgtc aactaggaag gaggtataaa atgaatgaac 61 atcaaaaaat tcgctaaaca agctacagtt cttacattca caacagctct tcttgctggc 121 ggcgctacac aagctttcgc taacgcccac attgtcatgg tcgacgcgta taaaccgacc 181 aaactggacg gtcacggcgt tggcgttccg ggtgtaggcg ttccgggcgt tggtgttccg 241 ggcgtaggcg aactggctca tattgttatg gtcgacgcgt acaaaccgac caaaacctct 301 gttccgggcg ttggcgttcc gggtgtaggc gttccgggcg aaggcggtct gctggatgcc 361 catattgtca tggtcgacgc gtataaaccg acgaaaacct ctgttccggg cgttggtgtt 421 ccgggcgaag gcgttccggg cgtaggtgtt ccgggcgttg gtgttccggg cggtgaactg 481 ctgatggcaa gcagcgaaga cgtcatcaag gaatttatgc ggttcaaggt acggatggaa 541 ggaagcgtaa acggccacga atttgaaatc gaaggcgaag gagaaggaag accttacgaa 601 ggaacgcaga cagcgaaact gaaagtcacg aagggaggac ctttaccgtt tgcttgggat 661 atccttagcc cgcaattcca atacggaagc aaggcatacg tcaaacatcc ggcagatatt 721 ccggactacc tgaaacttag cttcccggaa ggattcaagt gggaacgggt catgaacttc 781 gaagacggag gagtagtcac agtcacacaa gatagcagcc ttcaggacgg agaatttatt 841 tacaaggtaa aactgcgggg aacaaacttc ccgagcgacg gaccggtaat gcagaaaaag 901 acaatgggtt gggaagcaag cacagaaagg atgtatccgg aagacggagc acttaaaggc 961 gaaatcaaga tgcggctgaa gctgaaagac ggaggacatt acgacgcaga agtcaaaacg 1021 acgtacatgg cgaaaaaacc ggtccaactt ccgggagcat acaaaacgga catcaagctg 1081 gacatcacga gccacaacga agactacacg atcgtcgaac aatacgaacg ggcagaagga 1141 agacactcta caggagcaga tttccttaac tgctgccctg gctgctgcat ggaaccttaa 1201 t //