BBa_K1989042
1
BBa_K1989042
Triplespytag with mRFP, FlAsH-tag and signal peptide SacB
2016-10-11T11:00:00Z
2016-10-11T11:15:16Z
Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant.
The fused protein SacB-3A-mRFP-FlAsHtag possess both isopeptide bond forming function, uranyl-binding ability and secretion. Thus, using SacB-3A-mRFP-FlAsHtag as a part of hydrogel formation, we can obtain our multifunctional biomaterial.
false
false
_2456_
17187
17187
9
false
The length of the linker between spytag may affect the physical and chemical property of hydrogel. As several attributes of our target proteins, especially their folding state in cells, we were unable to rationally design or choose a signal peptide for each. Nevertheless, we could build a Signal Peptide Repertory to screen for the best SP candidate. Our ultimate goal was to select the most appropriate ones from a huge amount of signal peptides.
false
Yiming Dong
annotation2493172
1
RBS
range2493172
1
30
50
annotation2493180
1
Spytag-Spytag-Spytag-mRFP
range2493180
1
145
1158
annotation2493179
1
Signal peptide SacB
range2493179
1
55
141
annotation2493194
1
FlAsH-tag
range2493194
1
1162
1200
BBa_K1989042_sequence
1
tgaacttaagcaaaaggagagggacgcgtcaactaggaaggaggtataaaatgaatgaacatcaaaaaattcgctaaacaagctacagttcttacattcacaacagctcttcttgctggcggcgctacacaagctttcgctaacgcccacattgtcatggtcgacgcgtataaaccgaccaaactggacggtcacggcgttggcgttccgggtgtaggcgttccgggcgttggtgttccgggcgtaggcgaactggctcatattgttatggtcgacgcgtacaaaccgaccaaaacctctgttccgggcgttggcgttccgggtgtaggcgttccgggcgaaggcggtctgctggatgcccatattgtcatggtcgacgcgtataaaccgacgaaaacctctgttccgggcgttggtgttccgggcgaaggcgttccgggcgtaggtgttccgggcgttggtgttccgggcggtgaactgctgatggcaagcagcgaagacgtcatcaaggaatttatgcggttcaaggtacggatggaaggaagcgtaaacggccacgaatttgaaatcgaaggcgaaggagaaggaagaccttacgaaggaacgcagacagcgaaactgaaagtcacgaagggaggacctttaccgtttgcttgggatatccttagcccgcaattccaatacggaagcaaggcatacgtcaaacatccggcagatattccggactacctgaaacttagcttcccggaaggattcaagtgggaacgggtcatgaacttcgaagacggaggagtagtcacagtcacacaagatagcagccttcaggacggagaatttatttacaaggtaaaactgcggggaacaaacttcccgagcgacggaccggtaatgcagaaaaagacaatgggttgggaagcaagcacagaaaggatgtatccggaagacggagcacttaaaggcgaaatcaagatgcggctgaagctgaaagacggaggacattacgacgcagaagtcaaaacgacgtacatggcgaaaaaaccggtccaacttccgggagcatacaaaacggacatcaagctggacatcacgagccacaacgaagactacacgatcgtcgaacaatacgaacgggcagaaggaagacactctacaggagcagatttccttaactgctgccctggctgctgcatggaaccttaat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z