BBa_K1989043 1 BBa_K1989043 Triplespytag with mRFP, FlAsH-tag and signal peptide YjfA 2016-10-11T11:00:00Z 2016-10-11T11:35:49Z Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant. The fused protein YjfA-3A-mRFP-FlAsHtag possess both isopeptide bond forming function, uranyl-binding ability and secretion. Thus, using YjfA-3A-mRFP-FlAsHtag as a part of hydrogel formation, we can obtain our multifunctional biomaterial. false false _2456_ 17187 17187 9 false The length of the linker between spytag may affect the physical and chemical property of hydrogel. As several attributes of our target proteins, especially their folding state in cells, we were unable to rationally design or choose a signal peptide for each. Nevertheless, we could build a Signal Peptide Repertory to screen for the best SP candidate. Our ultimate goal was to select the most appropriate ones from a huge amount of signal peptides. false Yiming Dong annotation2493223 1 Spytag-Spytag-Spytag-mRFP range2493223 1 144 1157 annotation2493222 1 Signal peptide YjfA range2493222 1 57 140 annotation2493221 1 RBS range2493221 1 30 52 annotation2493224 1 FlAsH-tag range2493224 1 1161 1199 BBa_K1989043_sequence 1 tgaacttaagcaaaaggagagggacgcgtgtcatccaccctagggggtaacaatgaatgaaacgtcttttcatgaaagcttctcttgttcttttcgctgttgttttcgttttcgctgttaaaggcgctcctgctaaagctaacgcccacattgtcatggtcgacgcgtataaaccgaccaaactggacggtcacggcgttggcgttccgggtgtaggcgttccgggcgttggtgttccgggcgtaggcgaactggctcatattgttatggtcgacgcgtacaaaccgaccaaaacctctgttccgggcgttggcgttccgggtgtaggcgttccgggcgaaggcggtctgctggatgcccatattgtcatggtcgacgcgtataaaccgacgaaaacctctgttccgggcgttggtgttccgggcgaaggcgttccgggcgtaggtgttccgggcgttggtgttccgggcggtgaactgctgatggcaagcagcgaagacgtcatcaaggaatttatgcggttcaaggtacggatggaaggaagcgtaaacggccacgaatttgaaatcgaaggcgaaggagaaggaagaccttacgaaggaacgcagacagcgaaactgaaagtcacgaagggaggacctttaccgtttgcttgggatatccttagcccgcaattccaatacggaagcaaggcatacgtcaaacatccggcagatattccggactacctgaaacttagcttcccggaaggattcaagtgggaacgggtcatgaacttcgaagacggaggagtagtcacagtcacacaagatagcagccttcaggacggagaatttatttacaaggtaaaactgcggggaacaaacttcccgagcgacggaccggtaatgcagaaaaagacaatgggttgggaagcaagcacagaaaggatgtatccggaagacggagcacttaaaggcgaaatcaagatgcggctgaagctgaaagacggaggacattacgacgcagaagtcaaaacgacgtacatggcgaaaaaaccggtccaacttccgggagcatacaaaacggacatcaagctggacatcacgagccacaacgaagactacacgatcgtcgaacaatacgaacgggcagaaggaagacactctacaggagcagatttccttaactgctgccctggctgctgcatggaaccttaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z