LOCUS BBa_K1989046 1238 bp DNA linear 23-Dec-2024 DEFINITION Triplespytag with amilGFP, His-tag , FlAsH-tag and signal peptide PhoA ACCESSION BBa_K1989046 VERSION BBa_K1989046.1 FEATURES Location/Qualifiers CDS 130..377 /label=Spytag-Spytag-Spytag CDS 382..461 /label=Linker CDS 466..1164 /label=amilGFP misc_feature 91..129 /label=His-tag CDS 24..90 /label=Signal peptide PhoA misc_feature 1169..1207 /label=FlAsH-tag ORIGIN 1 tggtttaact ttaagaagga gatatacatg aaacagtcta ccatcgctct ggctctgctg 61 ccgctgctgt tcaccccggt taccaaagct atgaaaggtt cttctcatca tcatcatcat 121 cacgttgacg cccatattgt tatggtggat gcatataaac cgaccaaact ggatggtcat 181 ggtgtgggtg ttccgggtgt tggtgtgcct ggcgttggcg ttcctggtgt gggcgaactg 241 gcacatatcg tgatggttga tgcctacaaa cctacaaaaa ccagcgtgcc aggtgttgga 301 gtaccgggtg taggcgttcc aggtgaaggt ggtctgctgg atgcgcacat tgtaatggtt 361 gacgcgtata aaccaacgaa aaccagcgtt ccgggtgttg gtgttcctgg tgaaggtgtg 421 cctggtgtgg gtgtaccagg cgtaggtgtt ccaggtggtg aactgatgtc ttattcaaag 481 catggcatcg tacaagaaat gaagacgaaa taccatatgg aaggcagtgt caatggccat 541 gaatttacga tcgaaggtgt aggaactggg tacccttacg aagggaaaca gatgtccgaa 601 ttagtgatca tcaagcctgc gggaaaaccc cttccattct cctttgacat actgtcatca 661 gtctttcaat atggaaaccg ttgcttcaca aagtacccgg cagacatgcc tgactatttc 721 aagcaagcat tcccagatgg aatgtcatat gaaaggtcat ttctatttga ggatggagca 781 gttgctacag ccagctggaa cattcgtctc gaaggaaatt gcttcatcca caaatccatc 841 tttcatggcg taaactttcc cgctgatgga cccgtaatga aaaagaagac aattgactgg 901 gataagtcct tcgaaaaaat gactgtgtct aaagaggtgc taagaggtga cgtgactatg 961 tttcttatgc tcgaaggagg tggttctcac agatgccaat ttcactccac ttacaaaaca 1021 gagaagccgg tcacactgcc cccgaatcat gtcgtagaac atcaaattgt gaggaccgac 1081 cttggccaaa gtgcaaaagg ctttacagtc aagctggaag cacatgccgc ggctcatgtt 1141 aaccctttga aggttaaata ataattcctt cctgaactgc tgcccgggtt gctgcatgga 1201 accgtaacaa agcccgaaag gaagctgagt tggcttaa //