LOCUS       BBa_K1989046            1238 bp    DNA     linear       29-May-2024
DEFINITION  Triplespytag with amilGFP, His-tag , FlAsH-tag and signal peptide 
            PhoA
ACCESSION   BBa_K1989046
VERSION     BBa_K1989046.1  
FEATURES             Location/Qualifiers
     CDS             130..377
                     /label=Spytag-Spytag-Spytag
     CDS             382..461
                     /label=Linker
     CDS             466..1164
                     /label=amilGFP
     misc_feature    91..129
                     /label=His-tag
     CDS             24..90
                     /label=Signal peptide PhoA
     misc_feature    1169..1207
                     /label=FlAsH-tag
ORIGIN
        1 tggtttaact ttaagaagga gatatacatg aaacagtcta ccatcgctct ggctctgctg
       61 ccgctgctgt tcaccccggt taccaaagct atgaaaggtt cttctcatca tcatcatcat
      121 cacgttgacg cccatattgt tatggtggat gcatataaac cgaccaaact ggatggtcat
      181 ggtgtgggtg ttccgggtgt tggtgtgcct ggcgttggcg ttcctggtgt gggcgaactg
      241 gcacatatcg tgatggttga tgcctacaaa cctacaaaaa ccagcgtgcc aggtgttgga
      301 gtaccgggtg taggcgttcc aggtgaaggt ggtctgctgg atgcgcacat tgtaatggtt
      361 gacgcgtata aaccaacgaa aaccagcgtt ccgggtgttg gtgttcctgg tgaaggtgtg
      421 cctggtgtgg gtgtaccagg cgtaggtgtt ccaggtggtg aactgatgtc ttattcaaag
      481 catggcatcg tacaagaaat gaagacgaaa taccatatgg aaggcagtgt caatggccat
      541 gaatttacga tcgaaggtgt aggaactggg tacccttacg aagggaaaca gatgtccgaa
      601 ttagtgatca tcaagcctgc gggaaaaccc cttccattct cctttgacat actgtcatca
      661 gtctttcaat atggaaaccg ttgcttcaca aagtacccgg cagacatgcc tgactatttc
      721 aagcaagcat tcccagatgg aatgtcatat gaaaggtcat ttctatttga ggatggagca
      781 gttgctacag ccagctggaa cattcgtctc gaaggaaatt gcttcatcca caaatccatc
      841 tttcatggcg taaactttcc cgctgatgga cccgtaatga aaaagaagac aattgactgg
      901 gataagtcct tcgaaaaaat gactgtgtct aaagaggtgc taagaggtga cgtgactatg
      961 tttcttatgc tcgaaggagg tggttctcac agatgccaat ttcactccac ttacaaaaca
     1021 gagaagccgg tcacactgcc cccgaatcat gtcgtagaac atcaaattgt gaggaccgac
     1081 cttggccaaa gtgcaaaagg ctttacagtc aagctggaag cacatgccgc ggctcatgtt
     1141 aaccctttga aggttaaata ataattcctt cctgaactgc tgcccgggtt gctgcatgga
     1201 accgtaacaa agcccgaaag gaagctgagt tggcttaa
//