LOCUS       BBa_K1989047            1228 bp    DNA     linear       23-Dec-2024
DEFINITION  Triplespytag with amilGFP, His-tag , FlAsH-tag and signal peptide 
            LTIIB
ACCESSION   BBa_K1989047
VERSION     BBa_K1989047.1  
FEATURES             Location/Qualifiers
     CDS             24..80
                     /label=Signal peptide LTIIB
     misc_feature    81..119
                     /label=His-tag
     CDS             120..367
                     /label=Spytag-Spytag-Spytag
     CDS             372..451
                     /label=Linker
     CDS             456..1154
                     /label=amilGFP
     misc_feature    1159..1197
                     /label=FlAsH-tag
ORIGIN
        1 tggtttaact ttaagaagga gattctttca aaaaaatcat caaagctttc gttatcatgg
       61 ctgctctggt ttctgttcag atgaaaggtt cttctcatca tcatcatcat cacgttgacg
      121 cccatattgt tatggtggat gcatataaac cgaccaaact ggatggtcat ggtgtgggtg
      181 ttccgggtgt tggtgtgcct ggcgttggcg ttcctggtgt gggcgaactg gcacatatcg
      241 tgatggttga tgcctacaaa cctacaaaaa ccagcgtgcc aggtgttgga gtaccgggtg
      301 taggcgttcc aggtgaaggt ggtctgctgg atgcgcacat tgtaatggtt gacgcgtata
      361 aaccaacgaa aaccagcgtt ccgggtgttg gtgttcctgg tgaaggtgtg cctggtgtgg
      421 gtgtaccagg cgtaggtgtt ccaggtggtg aactgatgtc ttattcaaag catggcatcg
      481 tacaagaaat gaagacgaaa taccatatgg aaggcagtgt caatggccat gaatttacga
      541 tcgaaggtgt aggaactggg tacccttacg aagggaaaca gatgtccgaa ttagtgatca
      601 tcaagcctgc gggaaaaccc cttccattct cctttgacat actgtcatca gtctttcaat
      661 atggaaaccg ttgcttcaca aagtacccgg cagacatgcc tgactatttc aagcaagcat
      721 tcccagatgg aatgtcatat gaaaggtcat ttctatttga ggatggagca gttgctacag
      781 ccagctggaa cattcgtctc gaaggaaatt gcttcatcca caaatccatc tttcatggcg
      841 taaactttcc cgctgatgga cccgtaatga aaaagaagac aattgactgg gataagtcct
      901 tcgaaaaaat gactgtgtct aaagaggtgc taagaggtga cgtgactatg tttcttatgc
      961 tcgaaggagg tggttctcac agatgccaat ttcactccac ttacaaaaca gagaagccgg
     1021 tcacactgcc cccgaatcat gtcgtagaac atcaaattgt gaggaccgac cttggccaaa
     1081 gtgcaaaagg ctttacagtc aagctggaag cacatgccgc ggctcatgtt aaccctttga
     1141 aggttaaata ataattcctt cctgaactgc tgcccgggtt gctgcatgga accgtaacaa
     1201 agcccgaaag gaagctgagt tggcttaa
//