LOCUS       BBa_K1989048            1237 bp    DNA     linear       09-Jan-2025
DEFINITION  Triplespytag with amilGFP, His-tag , FlAsH-tag and signal peptide 
            PelB
ACCESSION   BBa_K1989048
VERSION     BBa_K1989048.1  
FEATURES             Location/Qualifiers
     CDS             24..89
                     /label=Signal peptide PelB
     misc_feature    90..128
                     /label=His-tag
     CDS             129..376
                     /label=Spytag-Spytag-Spytag
     CDS             381..460
                     /label=Linker
     CDS             465..1163
                     /label=amilGFP
     misc_feature    1168..1206
                     /label=FlAsH-tag
ORIGIN
        1 tggtttaact ttaagaagga gatatgaaat acctgctgcc gaccgctgct gctggtctgc
       61 tgctgctggc tgctcagccg gctatggcta tgaaaggttc ttctcatcat catcatcatc
      121 acgttgacgc ccatattgtt atggtggatg catataaacc gaccaaactg gatggtcatg
      181 gtgtgggtgt tccgggtgtt ggtgtgcctg gcgttggcgt tcctggtgtg ggcgaactgg
      241 cacatatcgt gatggttgat gcctacaaac ctacaaaaac cagcgtgcca ggtgttggag
      301 taccgggtgt aggcgttcca ggtgaaggtg gtctgctgga tgcgcacatt gtaatggttg
      361 acgcgtataa accaacgaaa accagcgttc cgggtgttgg tgttcctggt gaaggtgtgc
      421 ctggtgtggg tgtaccaggc gtaggtgttc caggtggtga actgatgtct tattcaaagc
      481 atggcatcgt acaagaaatg aagacgaaat accatatgga aggcagtgtc aatggccatg
      541 aatttacgat cgaaggtgta ggaactgggt acccttacga agggaaacag atgtccgaat
      601 tagtgatcat caagcctgcg ggaaaacccc ttccattctc ctttgacata ctgtcatcag
      661 tctttcaata tggaaaccgt tgcttcacaa agtacccggc agacatgcct gactatttca
      721 agcaagcatt cccagatgga atgtcatatg aaaggtcatt tctatttgag gatggagcag
      781 ttgctacagc cagctggaac attcgtctcg aaggaaattg cttcatccac aaatccatct
      841 ttcatggcgt aaactttccc gctgatggac ccgtaatgaa aaagaagaca attgactggg
      901 ataagtcctt cgaaaaaatg actgtgtcta aagaggtgct aagaggtgac gtgactatgt
      961 ttcttatgct cgaaggaggt ggttctcaca gatgccaatt tcactccact tacaaaacag
     1021 agaagccggt cacactgccc ccgaatcatg tcgtagaaca tcaaattgtg aggaccgacc
     1081 ttggccaaag tgcaaaaggc tttacagtca agctggaagc acatgccgcg gctcatgtta
     1141 accctttgaa ggttaaataa taattccttc ctgaactgct gcccgggttg ctgcatggaa
     1201 ccgtaacaaa gcccgaaagg aagctgagtt ggcttaa
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