LOCUS       BBa_K1989050            1220 bp    DNA     linear       26-Dec-2024
DEFINITION  Triplespytag with eforRed, His-tag , FlAsH-tag and signal peptide 
            PhoA
ACCESSION   BBa_K1989050
VERSION     BBa_K1989050.1  
FEATURES             Location/Qualifiers
     misc_feature    1151..1189
                     /label=FlAsH-tag
     CDS             24..90
                     /label=Signal peptide PhoA
     misc_feature    91..129
                     /label=His-tag
     CDS             130..377
                     /label=Spytag-Spytag-Spytag
     CDS             382..461
                     /label=Linker
     CDS             466..1146
                     /label=eforRed
ORIGIN
        1 tggtttaact ttaagaagga gatatacatg aaacagtcta ccatcgctct ggctctgctg
       61 ccgctgctgt tcaccccggt taccaaagct atgaaaggtt cttctcatca tcatcatcat
      121 cacgttgacg cccatattgt tatggtggat gcatataaac cgaccaaact ggatggtcat
      181 ggtgtgggtg ttccgggtgt tggtgtgcct ggcgttggcg ttcctggtgt gggcgaactg
      241 gcacatatcg tgatggttga tgcctacaaa cctacaaaaa ccagcgtgcc aggtgttgga
      301 gtaccgggtg taggcgttcc aggtgaaggt ggtctgctgg atgcgcacat tgtaatggtt
      361 gacgcgtata aaccaacgaa aaccagcgtt ccgggtgttg gtgttcctgg tgaaggtgtg
      421 cctggtgtgg gtgtaccagg cgtaggtgtt ccaggtggtg aactgatgtc agtgattaag
      481 caggtaatga agaccaagtt gcaccttgag ggcactgtca atggccatga ttttacgatc
      541 gagggtaaag gtgaaggcaa gccgtacgaa gggttacagc acatgaaaat gacagtcacc
      601 aaaggcgcgc ctctgccgtt ttccgttcat attcttacac ctagccacat gtatggaagc
      661 aaaccgttta ataagtatcc agcggatatc ccagactacc acaaacagtc ttttcccgaa
      721 ggtatgtctt gggagcggtc gatgattttt gaagatggtg gcgtatgcac cgccagtaat
      781 cactccagca taaacttgca agagaactgt ttcatctatg atgttaaatt tcatggtgtg
      841 aacctgcctc cggatgggcc cgtaatgcaa aaaaccattg ctggatggga gccgagcgtg
      901 gaaacactgt acgtgcgtga cgggatgtta aaaagtgaca ctgcaatggt ttttaaactg
      961 aaaggaggcg gtcatcatcg tgttgatttc aaaacgacgt ataaagccaa aaaacctgtc
     1021 aagctgccag aatttcattt cgttgaacat cgcctggaac tgaccaaaca cgataaagat
     1081 ttcacaactt gggaccagca ggaggcagcc gaaggccatt tctcaccgct gccgaaggct
     1141 ctcccattcc ttcctgaact gctgcccggg ttgctgcatg gaaccgtaac aaagcccgaa
     1201 aggaagctga gttggcttaa
//