LOCUS BBa_K1989051 1210 bp DNA linear 23-Dec-2024 DEFINITION Triplespytag with eforRed, His-tag , FlAsH-tag and signal peptide LTIIB ACCESSION BBa_K1989051 VERSION BBa_K1989051.1 FEATURES Location/Qualifiers CDS 24..80 /label=Signal peptide LTIIB misc_feature 81..119 /label=His-tag CDS 120..367 /label=Spytag-Spytag-Spytag CDS 372..451 /label=Linker CDS 456..1136 /label=eforRed misc_feature 1141..1179 /label=FlAsH-tag ORIGIN 1 tggtttaact ttaagaagga gattctttca aaaaaatcat caaagctttc gttatcatgg 61 ctgctctggt ttctgttcag atgaaaggtt cttctcatca tcatcatcat cacgttgacg 121 cccatattgt tatggtggat gcatataaac cgaccaaact ggatggtcat ggtgtgggtg 181 ttccgggtgt tggtgtgcct ggcgttggcg ttcctggtgt gggcgaactg gcacatatcg 241 tgatggttga tgcctacaaa cctacaaaaa ccagcgtgcc aggtgttgga gtaccgggtg 301 taggcgttcc aggtgaaggt ggtctgctgg atgcgcacat tgtaatggtt gacgcgtata 361 aaccaacgaa aaccagcgtt ccgggtgttg gtgttcctgg tgaaggtgtg cctggtgtgg 421 gtgtaccagg cgtaggtgtt ccaggtggtg aactgatgtc agtgattaag caggtaatga 481 agaccaagtt gcaccttgag ggcactgtca atggccatga ttttacgatc gagggtaaag 541 gtgaaggcaa gccgtacgaa gggttacagc acatgaaaat gacagtcacc aaaggcgcgc 601 ctctgccgtt ttccgttcat attcttacac ctagccacat gtatggaagc aaaccgttta 661 ataagtatcc agcggatatc ccagactacc acaaacagtc ttttcccgaa ggtatgtctt 721 gggagcggtc gatgattttt gaagatggtg gcgtatgcac cgccagtaat cactccagca 781 taaacttgca agagaactgt ttcatctatg atgttaaatt tcatggtgtg aacctgcctc 841 cggatgggcc cgtaatgcaa aaaaccattg ctggatggga gccgagcgtg gaaacactgt 901 acgtgcgtga cgggatgtta aaaagtgaca ctgcaatggt ttttaaactg aaaggaggcg 961 gtcatcatcg tgttgatttc aaaacgacgt ataaagccaa aaaacctgtc aagctgccag 1021 aatttcattt cgttgaacat cgcctggaac tgaccaaaca cgataaagat ttcacaactt 1081 gggaccagca ggaggcagcc gaaggccatt tctcaccgct gccgaaggct ctcccattcc 1141 ttcctgaact gctgcccggg ttgctgcatg gaaccgtaac aaagcccgaa aggaagctga 1201 gttggcttaa //