BBa_K1989053 1 BBa_K1989053 Triple spytag with CBP and His-tag 2016-10-11T11:00:00Z 2016-10-18T01:52:42Z Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant. The fused protein Histag-3A-CBP possess both isopeptide bond forming function and cadmium-binding ability . Thus, using Histag-3A-CBP as a part of hydrogel formation, we can obtain our multifunctional biomaterial. false false _2456_ 17187 17187 9 false The length of the linker between spytag may affect the physical and chemical property of hydrogel. As several attributes of our target proteins, especially their folding state in cells, we were unable to rationally design or choose a signal peptide for each. Nevertheless, we could build a Signal Peptide Repertory to screen for the best SP candidate. Our ultimate goal was to select the most appropriate ones from a huge amount of signal peptides. false Yiming Dong annotation2493814 1 Linker range2493814 1 320 399 annotation2493812 1 Spytag-Spytag-Spytag range2493812 1 68 315 annotation2493811 1 His-tag range2493811 1 29 67 annotation2493816 1 CBP range2493816 1 407 643 BBa_K1989053_sequence 1 tggtttaactttaagaaggagatataccatgaaaggttcttctcatcatcatcatcatcacgttgacgcccatattgttatggtggatgcatataaaccgaccaaactggatggtcatggtgtgggtgttccgggtgttggtgtgcctggcgttggcgttcctggtgtgggcgaactggcacatatcgtgatggttgatgcctacaaacctacaaaaaccagcgtgccaggtgttggagtaccgggtgtaggcgttccaggtgaaggtggtctgctggatgcgcacattgtaatggttgacgcgtataaaccaacgaaaaccagcgttccgggtgttggtgttcctggtgaaggtgtgcctggtgtgggtgtaccaggcgtaggtgttccaggtggtgaactggggctgcgtgactctcctgacgattcctgcggttctgtaaacgctctgatcgacgaacatatcgagcacgtgcaagcgcgtatcgacggtctggtagcactacaggagcagctggtggaactgcgccgtcgttgtaatgcgcagggtgcggaatgcgctattctacagcaactggaaaccaacggtgccgtgagcgtcccggaaaccgaacactcccacgttggtcgctctcacggccactaataacaaagcccgaaaggaagctgagttggcttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z