BBa_K1989054
1
BBa_K1989054
Triple spytag with MBP and His-tag
2016-10-11T11:00:00Z
2016-10-12T05:55:10Z
Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant.
The fused protein Histag-3A-MBP possess both isopeptide bond forming function and mercury-binding ability . Thus, using Histag-3A-MBP as a part of hydrogel formation, we can obtain our multifunctional biomaterial.
false
false
_2456_
17187
17187
9
false
The length of the linker between spytag may affect the physical and chemical property of hydrogel. As several attributes of our target proteins, especially their folding state in cells, we were unable to rationally design or choose a signal peptide for each. Nevertheless, we could build a Signal Peptide Repertory to screen for the best SP candidate. Our ultimate goal was to select the most appropriate ones from a huge amount of signal peptides.
false
Yiming Dong
annotation2493822
1
Linker
range2493822
1
320
399
annotation2493823
1
MBP
range2493823
1
416
745
annotation2493820
1
His-tag
range2493820
1
29
67
annotation2493821
1
Spytag-Spytag-Spytag
range2493821
1
68
315
BBa_K1989054_sequence
1
tggtttaactttaagaaggagatataccatgaaaggttcttctcatcatcatcatcatcacgttgacgcccatattgttatggtggatgcatataaaccgaccaaactggatggtcatggtgtgggtgttccgggtgttggtgtgcctggcgttggcgttcctggtgtgggcgaactggcacatatcgtgatggttgatgcctacaaacctacaaaaaccagcgtgccaggtgttggagtaccgggtgtaggcgttccaggtgaaggtggtctgctggatgcgcacattgtaatggttgacgcgtataaaccaacgaaaaccagcgttccgggtgttggtgttcctggtgaaggtgtgcctggtgtgggtgtaccaggcgtaggtgttccaggtggtgaactgggagtcgacgggacacactgcgaggaggccagcagcctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgttgatcgcgtcactacagggatcctcaggcacccactgcgaggaggccagcagcctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgagcgcttggagctaataacaaagcccgaaaggaagctgagttggcttaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z