LOCUS       BBa_K199013              940 bp    DNA     linear       27-Dec-2024
DEFINITION  pLacIQ1-RBS32-GFP-TT
ACCESSION   BBa_K199013
VERSION     BBa_K199013.1  
FEATURES             Location/Qualifiers
     promoter        12..17
                     /label=-35
     promoter        37..41
                     /label=-10
     promoter        1..56
                     /label=BBa_K091112
     RBS             65..77
                     /label=RBS-3\Weak
     RBS             71..74
                     /label=RBS
     misc_feature    65..77
                     /label=BBa_B0032
     RBS             65..77
                     /label=BBa_B0032
     CDS             84..803
                     /label=GFP protein
     CDS             84..803
                     /label=BBa_E0040
     stem_loop       823..866
                     /label=stem_loop
     misc_feature    812..891
                     /label=BBa_B0010
     terminator      812..891
                     /label=BBa_B0010
     stem_loop       907..926
                     /label=T7 TE
     misc_feature    933..933
                     /label=stop
     polyA_site      927..940
                     /label=polya
     misc_feature    900..940
                     /label=BBa_B0012
     terminator      900..940
                     /label=BBa_B0012
ORIGIN
        1 agcggcatgc atttacgttg acaccacctt tcgcggtatg gcatgatagc gcccggtact
       61 agagtcacac aggaaagtac tagatgcgta aaggagaaga acttttcact ggagttgtcc
      121 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
      181 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
      241 tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga
      301 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg
      361 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
      421 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
      481 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
      541 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
      601 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
      661 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
      721 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
      781 tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag
      841 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      901 cacactggct caccttcggg tgggcctttc tgcgtttata
//