BBa_J44001
1
BBa_J44001
Reverse RBS (RBS<sub>rev</sub>) -- corresponds to BBa_B0030
2006-08-01T11:00:00Z
2015-08-31T04:08:48Z
Cloned from synthetic oligos
Released HQ 2013
Reverse version of RBS BBa_B0030
false
false
_61_71_
0
606
61
In stock
true
Repeats in oligos caused unusual products during cloning
true
Todd Eckdahl
annotation1893199
1
Reverse RBS
range1893199
1
1
15
BBa_K199021
1
BBa_K199021
RBS-RFP (all inverted)
2009-07-07T11:00:00Z
2015-05-08T01:11:18Z
Ligation of existing parts
This part is oriented opposite to typical BioBrick arrangement in terms of placement of BioBrick prefix and suffix. This part can be ligated to a promoter to measure promoter activity.
false
false
_295_
0
5395
9
It's complicated
false
none
false
Kin Lau
component2010329
1
BBa_J44001
component2010327
1
BBa_J31008
annotation2010329
1
BBa_J44001
range2010329
1
687
701
annotation2010327
1
BBa_J31008
range2010327
1
1
678
BBa_J31008
1
mRFPr
RFP reverse
2007-02-15T12:00:00Z
2015-08-31T04:08:45Z
RFP reverse was PCR amplified from mRFP (BBaE1010).
The mRFP coding sequence in the reverse orientation. This part requires a reverse RBS and reverse promoter to be expressed
false
false
_61_
0
1144
61
It's complicated
false
RFP reverse was PCR amplified from mRFP (BBaE1010) using the following primers:
5'-atgcactagtatggcttcctccgaagacgt "Spe mRFP F"
5'-gcattctagattaagcaccggtggagtgac "Xba mRFP R"
false
Karmella Haynes
annotation1911764
1
Spe mRFP F
range1911764
1
659
678
annotation1911761
1
mRFP reverse
range1911761
1
1
678
annotation1911765
1
Xba mRFP R
range1911765
1
1
20
BBa_K199021_sequence
1
ttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccattactagagtttctcctctttaat
BBa_J44001_sequence
1
tttctcctctttaat
BBa_J31008_sequence
1
ttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z