LOCUS       BBa_K199026             1277 bp    DNA     linear       22-Dec-2024
DEFINITION  Tetrev-RBSrev-pLux
ACCESSION   BBa_K199026
VERSION     BBa_K199026.1  
FEATURES             Location/Qualifiers
     CDS             1..1191
                     /label=tetA(C) backwards
     misc_feature    1..3
                     /label=stop
     primer_bind     1..20
                     /label=Rev primer J31006
     primer_bind     1168..1191
                     /label=Fwd primer J31006
     CDS             1..1191
                     /label=BBa_J31006
     RBS             1200..1214
                     /label=Reverse RBS
     RBS             1200..1214
                     /label=BBa_J44001
     misc_feature    1223..1242
                     /label=LuxR/HSL
     promoter        1242..1247
                     /label=-35
     promoter        1264..1269
                     /label=-10
     misc_feature    1275..1275
                     /label=start
     misc_feature    1223..1277
                     /label=BBa_R0062
     promoter        1223..1277
                     /label=BBa_R0062
ORIGIN
        1 ttaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta
       61 tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat
      121 cgccgtgacg atcagcggtc caatgatcga agttaggctg gtaagagccg cgagcgatcc
      181 ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg
      241 catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt
      301 cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg
      361 cttctcgccg aaacgtttgg tggcggggcc agtgacgaag gcttgagcga gggcgtgcaa
      421 gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc
      481 gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac
      541 agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt
      601 gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca
      661 gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga
      721 gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
      781 gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc
      841 gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat
      901 ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc
      961 gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag
     1021 aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga
     1081 atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc
     1141 atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca ttactagagt
     1201 ttctcctctt taattactag agacctgtag gatcgtacag gtttacgcaa gaaaatggtt
     1261 tgttatagtc gaataaa
//