BBa_K199035
1
BBa_K199035
Tet Resistance with CGGUC Addition
2009-07-12T11:00:00Z
2015-05-08T01:11:19Z
The template of the gene comes from Part BBa_J31007. We designed a forward primer that included ATG, the 5-bp addition CGGTC and the first 20 nucleotides on the 5' end of Tet after ATG. This made the edited 5' sequence for our Tet.
This Tet reporter gene is a modification of BBa_J31007; it has a 5-bp addition, CGGUC, after the start codon and before the rest of the gene. This is used in conjunction with the CGGUC tRNA (Part K199028). These CGGUC tRNAs suppress the frameshift caused by this 5-bp addition in the reporter.
false
false
_295_
0
5117
9
It's complicated
false
We positioned the start codon, ATG, before the 5-bp codon and continued with the rest of the Tet gene. This way, the RBS wouldn't translate the reporter gene without reading over the 5-base codon first. In order to ligate our edited 5' sequence to the unchanged downstream portion of the Tet gene, we utilized the restriction enzyme BamHI that cuts at a single site 290-bp into BBa_J31007.
false
Alyndria Thompson
annotation2011264
1
5-bp CGGUC Addition
range2011264
1
4
9
annotation2011265
1
BamH1 restriciton site
range2011265
1
295
300
BBa_K199035_sequence
1
atgcggtcaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z