LOCUS       BBa_K199044             1272 bp    DNA     linear       26-Dec-2024
DEFINITION  pLux-RBS-Tet
ACCESSION   BBa_K199044
VERSION     BBa_K199044.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..1272
                     /label=tetA(C) forward
     primer_bind     82..105
                     /label=Fwd primer J31007
     primer_bind     1253..1272
                     /label=Rev primer J31007
     misc_feature    1270..1272
                     /label=stop
     CDS             82..1272
                     /label=BBa_J31007
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatgaaatct aacaatgcgc tcatcgtcat cctcggcacc
      121 gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg cctcttgcgg
      181 gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc gctatatgcg
      241 ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt tggccgccgc
      301 ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat ggcgaccaca
      361 cccgtcctgt ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
      421 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
      481 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
      541 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
      601 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc
      661 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
      721 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg
      781 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg
      841 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggccccgc caccaaacgt
      901 ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg
      961 ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc
     1021 ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag
     1081 ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat tggaccgctg
     1141 atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta
     1201 ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc
     1261 acctcgacct aa
//