LOCUS       BBa_K199049             1023 bp    DNA     linear       27-Dec-2024
DEFINITION  pLac+RBS+CAT+TT on pSB1AK3
ACCESSION   BBa_K199049
VERSION     BBa_K199049.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..886
                     /label=CmR gene
     CDS             227..886
                     /label=BBa_J31005
     stem_loop       906..949
                     /label=stem_loop
     misc_feature    895..974
                     /label=BBa_B0010
     terminator      895..974
                     /label=BBa_B0010
     stem_loop       990..1009
                     /label=T7 TE
     misc_feature    1016..1016
                     /label=stop
     polyA_site      1010..1023
                     /label=polya
     misc_feature    983..1023
                     /label=BBa_B0012
     terminator      983..1023
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg agaaaaaaat
      241 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt
      301 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt
      361 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg
      421 cctgatgaat gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg
      481 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct
      541 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc
      601 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt
      661 ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa
      721 cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat
      781 gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct
      841 taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatact agagccaggc
      901 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      961 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1021 ata
//