LOCUS       BBa_K199054             1755 bp    DNA     linear       22-Nov-2024
DEFINITION  RFPrev-RBSrev-pOmpC-pLuxrev-RBS-GFP-TT
ACCESSION   BBa_K199054
VERSION     BBa_K199054.1  
FEATURES             Location/Qualifiers
     CDS             1..678
                     /label=mRFP reverse
     primer_bind     659..678
                     /label=Spe mRFP F
     primer_bind     1..20
                     /label=Xba mRFP R
     misc_feature    1..678
                     /label=BBa_J31008
     RBS             687..701
                     /label=Reverse RBS
     RBS             687..701
                     /label=BBa_J44001
     promoter        710..808
                     /label=BBa_K199017
     promoter        817..871
                     /label=BBa_K199052
     RBS             880..892
                     /label=RBS-3\Weak
     RBS             886..889
                     /label=RBS
     misc_feature    880..892
                     /label=BBa_B0032
     RBS             880..892
                     /label=BBa_B0032
     CDS             899..1618
                     /label=GFP protein
     CDS             899..1618
                     /label=BBa_E0040
     stem_loop       1638..1681
                     /label=stem_loop
     misc_feature    1627..1706
                     /label=BBa_B0010
     terminator      1627..1706
                     /label=BBa_B0010
     stem_loop       1722..1741
                     /label=T7 TE
     misc_feature    1748..1748
                     /label=stop
     polyA_site      1742..1755
                     /label=polya
     misc_feature    1715..1755
                     /label=BBa_B0012
     terminator      1715..1755
                     /label=BBa_B0012
ORIGIN
        1 ttaagcaccg gtggagtgac gaccttcagc acgttcgtac tgttcaacga tggtgtagtc
       61 ttcgttgtgg gaggtgatgt ccagtttgat gtcggttttg taagcacccg gcagctgaac
      121 cggtttttta gccatgtagg tggttttaac ttcagcgtcg tagtgaccac cgtctttcag
      181 tttcagacgc attttgattt cacctttcag agcaccgtct tccgggtaca tacgttcggt
      241 ggaagcttcc caacccatgg tttttttctg cataaccgga ccgtcggacg ggaagttggt
      301 accacgcagt ttaactttgt agatgaactc accgtcttgc agggaggagt cctgggtaac
      361 ggtaacaaca ccaccgtctt cgaagttcat aacacgttcc catttgaaac cttccgggaa
      421 ggacagtttc aggtagtccg ggatgtcagc cgggtgttta acgtaagctt tggaaccgta
      481 ctggaactgc ggggacagga tgtcccaagc gaacggcagc ggaccacctt tggtaacttt
      541 cagtttagcg gtctgggtac cttcgtacgg acgaccttca ccttcacctt cgatttcgaa
      601 ctcgtgaccg ttaacggaac cttccatacg aactttgaaa cgcatgaact ctttgataac
      661 gtcttcggag gaagccatta ctagagtttc tcctctttaa ttactagagt ttacattttg
      721 aaacatctat agcgataaat gaaacatctt aaaagtttta gtatcatatt cgtgttggat
      781 tattctgcat ttttggggag aatggactta ctagagttta ttcgactata acaaaccatt
      841 ttcttgcgta aacctgtacg atcctacagg ttactagagt cacacaggaa agtactagat
      901 gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
      961 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
     1021 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt
     1081 cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca
     1141 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa
     1201 agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa
     1261 tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt
     1321 ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat
     1381 caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca
     1441 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct
     1501 gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct
     1561 tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca aataataata
     1621 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1681 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1741 ctttctgcgt ttata
//