BBa_K199056
type: ComponentDefinition
persistent identity: BBa K199056
display id: BBa_K199056
version: 1
was generated by: iGEM to SBOL conversion
description: CCAUC tRNA (9-bp Anticodon) with RFP Reporter
created: 2009-08-04T11:00:00Z
modified: 2015-05-08T01:11:19Z
mutable provenance: From the BioBrick Parts Registry, and mutation of the RFP reporter, E1010, to add the 5-bp insertion.
owned by: myers
owned by: james
mutable description: Suppressor tRNA expressed by the medium-weak promoter, pBad, upstream of RFP with a 5-bp insertion, CCAUC. The tRNA is designed to suppress the insertion, allowing the appropriate translation of functional RFP.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _295_
m user id: 0
owner id: 5112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: See the following parts for more information: K199007, K199031 and K199009.
star: false
creator: Olivia Ho-Shing
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J04500
component: BBa_K199031
component: BBa_K199009
sequence annotation: BBa_K199031
sequence annotation: BBa_J04500
sequence annotation: BBa_K199009
sequence: https://synbiohub.org/public/igem/BBa_K199056_sequence/1

BBa_J04500
type: Component
persistent identity: component2222323
display id: component2222323
version: 1
top level: BBa_K199056
definition: BBa_J04500
access: public

BBa_K199031
type: Component
persistent identity: component2222314
display id: component2222314
version: 1
top level: BBa_K199056
definition: BBa_K199031
access: public

BBa_K199009
type: Component
persistent identity: component2222328
display id: component2222328
version: 1
top level: BBa_K199056
definition: BBa_K199009
access: public

BBa_K199031
type: SequenceAnnotation
persistent identity: annotation2222314
display id: annotation2222314
version: 1
top level: BBa_K199056
location: https://synbiohub.org/public/igem/BBa_K199056/annotation2222314/range2222314/1
component: BBa_K199031

https://synbiohub.org/public/igem/BBa_K199056/annotation2222314/range2222314/1
type: Range
persistent identity: range2222314
display id: range2222314
version: 1
top level: BBa_K199056
direction: direction/reverse
start: 1
end: 281
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2222323
display id: annotation2222323
version: 1
top level: BBa_K199056
location: https://synbiohub.org/public/igem/BBa_K199056/annotation2222323/range2222323/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K199056/annotation2222323/range2222323/1
type: Range
persistent identity: range2222323
display id: range2222323
version: 1
top level: BBa_K199056
direction: direction/reverse
start: 290
end: 509
orientation: inline

BBa_K199009
type: SequenceAnnotation
persistent identity: annotation2222328
display id: annotation2222328
version: 1
top level: BBa_K199056
location: https://synbiohub.org/public/igem/BBa_K199056/annotation2222328/range2222328/1
component: BBa_K199009

https://synbiohub.org/public/igem/BBa_K199056/annotation2222328/range2222328/1
type: Range
persistent identity: range2222328
display id: range2222328
version: 1
top level: BBa_K199056
direction: direction/reverse
start: 516
end: 1226
orientation: inline

BBa_K199009
type: ComponentDefinition
persistent identity: BBa K199009
display id: BBa_K199009
version: 1
was generated by: iGEM to SBOL conversion
description: RFP with CCAUC Addition
created: 2009-06-28T11:00:00Z
modified: 2015-05-08T01:11:18Z
mutable provenance: The template of the gene comes from BioBrick Part E1010. We designed a forward primer that included ATG, the 5-bp addition CCATC and the first 20 nucleotides on the 5' end of RFP after ATG. This made the edited 5' sequence for our RFP.
owned by: james
owned by: myers
mutable description: This RFP reporter gene has a 5-bp addition, CCAUC, after the start codon and before the rest of the gene. This is used in conjunction with the CCAUC tRNA (Parts K199007 and K199008). These CCAUC tRNAs suppress the frameshift caused by this 5-bp addition in the reporter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _295_
m user id: 0
owner id: 5112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We positioned the start codon, ATG, before the 5-bp codon and continued with the rest of the RFP gene. This way, the RBS wouldn't translate the reporter gene without reading over the 5-base codon first. In order to ligate our edited 5' sequence to the unchanged downstream portion of the RFP gene, we utilized the restriction enzyme NcoI that cuts at a single site 419-bp into BBa_E1010.
star: false
creator: Olivia Ho-Shing
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: 5-bp CCAUC Addition
sequence annotation: NcoI Restriction Site
sequence annotation: Double Stop
sequence annotation: 25 bp addition
sequence: https://synbiohub.org/public/igem/BBa_K199009_sequence/1

5-bp CCAUC Addition
type: SequenceAnnotation
persistent identity: annotation2006991
display id: annotation2006991
version: 1
top level: BBa_K199009
location: https://synbiohub.org/public/igem/BBa_K199009/annotation2006991/range2006991/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K199009/annotation2006991/range2006991/1
type: Range
persistent identity: range2006991
display id: range2006991
version: 1
top level: BBa_K199009
direction: direction/forward
start: 4
end: 8
orientation: inline

NcoI Restriction Site
type: SequenceAnnotation
persistent identity: annotation2006990
display id: annotation2006990
version: 1
top level: BBa_K199009
location: https://synbiohub.org/public/igem/BBa_K199009/annotation2006990/range2006990/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K199009/annotation2006990/range2006990/1
type: Range
persistent identity: range2006990
display id: range2006990
version: 1
top level: BBa_K199009
direction: direction/forward
start: 424
end: 429
orientation: inline

Double Stop
type: SequenceAnnotation
persistent identity: annotation2013715
display id: annotation2013715
version: 1
top level: BBa_K199009
location: https://synbiohub.org/public/igem/BBa_K199009/annotation2013715/range2013715/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K199009/annotation2013715/range2013715/1
type: Range
persistent identity: range2013715
display id: range2013715
version: 1
top level: BBa_K199009
direction: direction/forward
start: 681
end: 686
orientation: inline

25 bp addition
type: SequenceAnnotation
persistent identity: annotation2013327
display id: annotation2013327
version: 1
top level: BBa_K199009
location: https://synbiohub.org/public/igem/BBa_K199009/annotation2013327/range2013327/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K199009/annotation2013327/range2013327/1
type: Range
persistent identity: range2013327
display id: range2013327
version: 1
top level: BBa_K199009
direction: direction/forward
start: 687
end: 711
orientation: inline

BBa_K199031
type: ComponentDefinition
persistent identity: BBa K199031
display id: BBa_K199031
version: 1
was generated by: iGEM to SBOL conversion
description: pBad-CCAUC Suppressor tRNA (9-bp Anticodon)
created: 2009-07-12T11:00:00Z
modified: 2015-05-08T01:11:18Z
mutable provenance: The pBad promoter is from the BioBrick Parts registry. Suppressor tRNA was made by de novo synthesis from single-stranded oligos. Based on the paper by Anderson et al. 2002.
owned by: james
owned by: myers
mutable description: Causes low expression of a suppressor tRNA that suppresses a 5-bp codon CCAUC, Parts K199007 and K199008.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _295_
m user id: 0
owner id: 5112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We used the pBad promoter to transcribe lower levels of tRNA in a high copy plasmid, pSB1A2.
star: false
creator: Olivia Ho-Shing
type: DnaRegion
role: SO:0000834
role: partType/RNA
component: BBa_K199007
component: BBa_I13453
sequence annotation: BBa_I13453
sequence annotation: BBa_K199007
sequence: https://synbiohub.org/public/igem/BBa_K199031_sequence/1

BBa_K199007
type: Component
persistent identity: component2218714
display id: component2218714
version: 1
top level: BBa_K199031
definition: BBa_K199007
access: public

BBa_I13453
type: Component
persistent identity: component2218710
display id: component2218710
version: 1
top level: BBa_K199031
definition: BBa_I13453
access: public

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2218710
display id: annotation2218710
version: 1
top level: BBa_K199031
location: https://synbiohub.org/public/igem/BBa_K199031/annotation2218710/range2218710/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K199031/annotation2218710/range2218710/1
type: Range
persistent identity: range2218710
display id: range2218710
version: 1
top level: BBa_K199031
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_K199007
type: SequenceAnnotation
persistent identity: annotation2218714
display id: annotation2218714
version: 1
top level: BBa_K199031
location: https://synbiohub.org/public/igem/BBa_K199031/annotation2218714/range2218714/1
component: BBa_K199007

https://synbiohub.org/public/igem/BBa_K199031/annotation2218714/range2218714/1
type: Range
persistent identity: range2218714
display id: range2218714
version: 1
top level: BBa_K199031
direction: direction/reverse
start: 139
end: 281
orientation: inline

BBa_J04500
type: ComponentDefinition
persistent identity: BBa J04500
display id: BBa_J04500
version: 1
was generated by: iGEM to SBOL conversion
description: IPTG inducible promoter with RBS
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: R0010.B0034
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 326
owning group id: 16
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Kristen DeCelle
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0034
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J04500_sequence/1

BBa_B0034
type: Component
persistent identity: component1508159
display id: component1508159
version: 1
top level: BBa_J04500
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component1508149
display id: component1508149
version: 1
top level: BBa_J04500
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1508149
display id: annotation1508149
version: 1
top level: BBa_J04500
location: https://synbiohub.org/public/igem/BBa_J04500/annotation1508149/range1508149/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04500/annotation1508149/range1508149/1
type: Range
persistent identity: range1508149
display id: range1508149
version: 1
top level: BBa_J04500
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1508159
display id: annotation1508159
version: 1
top level: BBa_J04500
location: https://synbiohub.org/public/igem/BBa_J04500/annotation1508159/range1508159/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04500/annotation1508159/range1508159/1
type: Range
persistent identity: range1508159
display id: range1508159
version: 1
top level: BBa_J04500
direction: direction/forward
start: 209
end: 220
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

BBa_K199007
type: ComponentDefinition
persistent identity: BBa K199007
display id: BBa_K199007
version: 1
was generated by: iGEM to SBOL conversion
description: CCAUC Suppressor tRNA (9-bp Anticodon and Produces Serine)
created: 2009-06-28T11:00:00Z
modified: 2015-05-08T01:11:18Z
mutable provenance: De Novo synthesis from oligos. Based on the paper by Anderson et al. (http://gcat.davidson.edu/GcatWiki/images/6/6f/AndersonSchultz2002ChemBiol.pdf)
owned by: james
owned by: myers
mutable description: This gene encodes for a 5 base pair tRNA suppressor that suppresses CCAUC, which would normally cause a frame shift mutation.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _295_
m user id: 0
owner id: 5112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We used the 9 base anticodon loop that is complementary to the 5 base pair codon CCAUC.
star: false
creator: Olivia Ho-Shing
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: 5' Context
sequence annotation: Anticodon Loop
sequence annotation: 3' Context
sequence: https://synbiohub.org/public/igem/BBa_K199007_sequence/1

5' Context
type: SequenceAnnotation
persistent identity: annotation2006981
display id: annotation2006981
version: 1
top level: BBa_K199007
location: https://synbiohub.org/public/igem/BBa_K199007/annotation2006981/range2006981/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K199007/annotation2006981/range2006981/1
type: Range
persistent identity: range2006981
display id: range2006981
version: 1
top level: BBa_K199007
direction: direction/forward
start: 1
end: 11
orientation: inline

Anticodon Loop
type: SequenceAnnotation
persistent identity: annotation2006982
display id: annotation2006982
version: 1
top level: BBa_K199007
location: https://synbiohub.org/public/igem/BBa_K199007/annotation2006982/range2006982/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K199007/annotation2006982/range2006982/1
type: Range
persistent identity: range2006982
display id: range2006982
version: 1
top level: BBa_K199007
direction: direction/forward
start: 44
end: 52
orientation: inline

3' Context
type: SequenceAnnotation
persistent identity: annotation2006983
display id: annotation2006983
version: 1
top level: BBa_K199007
location: https://synbiohub.org/public/igem/BBa_K199007/annotation2006983/range2006983/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K199007/annotation2006983/range2006983/1
type: Range
persistent identity: range2006983
display id: range2006983
version: 1
top level: BBa_K199007
direction: direction/forward
start: 104
end: 143
orientation: inline

https://synbiohub.org/public/igem/BBa_K199056_sequence/1
type: Sequence
persistent identity: BBa K199056 sequence
display id: BBa_K199056_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagggatccaattcggagagatgccggagcggctgaacggaccggtgtgatggaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagcttactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgccatcgcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199009_sequence/1
type: Sequence
persistent identity: BBa K199009 sequence
display id: BBa_K199009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgccatcgcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199031_sequence/1
type: Sequence
persistent identity: BBa K199031 sequence
display id: BBa_K199031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagggatccaattcggagagatgccggagcggctgaacggaccggtgtgatggaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J04500_sequence/1
type: Sequence
persistent identity: BBa J04500 sequence
display id: BBa_J04500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199007_sequence/1
type: Sequence
persistent identity: BBa K199007 sequence
display id: BBa_K199007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggatccaattcggagagatgccggagcggctgaacggaccggtgtgatggaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z