LOCUS       BBa_K199057             1227 bp    DNA     linear       22-May-2024
DEFINITION  CCAUC tRNA (10-bp Anticodon) with RFP Reporter
ACCESSION   BBa_K199057
VERSION     BBa_K199057.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    139..149
                     /label=5' Context
     misc_feature    182..191
                     /label=Anticodon Loop
     misc_feature    243..282
                     /label=3' Context
     misc_feature    139..282
                     /label=BBa_K199008
     CDS             291..378
                     /label=end of LacI coding region (inactive)
     misc_feature    291..490
                     /label=BBa_R0010
     misc_feature    379..416
                     /label=CAP binding site
     promoter        427..432
                     /label=-35
     promoter        451..456
                     /label=-10
     misc_feature    456..490
                     /label=LacI binding site
     misc_feature    463..463
                     /label=start
     promoter        291..490
                     /label=BBa_R0010
     misc_feature    503..506
                     /label=conserved
     RBS             499..510
                     /label=BBa_B0034
     misc_feature    940..945
                     /label=NcoI Restriction Site
     misc_feature    520..524
                     /label=5-bp CCAUC Addition
     misc_feature    1203..1227
                     /label=25 bp addition
     misc_feature    1197..1202
                     /label=Double Stop
     misc_feature    517..1227
                     /label=BBa_K199009
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagaggg atccaattcg gagagatgcc ggagcggctg aacggaccgg
      181 ttttgatgga gaccggagta ggggcaactc taccgggggt tcaaatcccc ctctctccgc
      241 cactgcatat ccttagcgaa agctaaggat tttttttaag cttactagag caatacgcaa
      301 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
      361 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
      421 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
      481 atttcacaca tactagagaa agaggagaaa tactagatgc catcgcttcc tccgaagacg
      541 ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt
      601 tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga
      661 aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt
      721 acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct
      781 tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg
      841 ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta
      901 ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca
      961 ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac
     1021 tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg
     1081 ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag
     1141 actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat
     1201 aacgctgata gtgctagtgt agatcgc
//