##gff-version 3
##sequence-region BBa_K199057 1 1227
BBa_K199057	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453
BBa_K199057	.	engineered_region	517	1227	.	+	0	ID=BBa_K199009;Name=BBa_K199009
BBa_K199057	.	sequence_feature	1203	1227	.	+	0	ID=annotation2013327;Name=25 bp addition;Parent=BBa_K199009
BBa_K199057	.	sequence_alteration	520	524	.	+	0	ID=annotation2006991;Name=5-bp CCAUC Addition;Parent=BBa_K199009
BBa_K199057	.	sequence_feature	1197	1202	.	+	0	ID=annotation2013715;Name=Double Stop;Parent=BBa_K199009
BBa_K199057	.	sequence_feature	940	945	.	+	0	ID=annotation2006990;Name=NcoI Restriction Site;Parent=BBa_K199009
BBa_K199057	.	mature_transcript_region	139	282	.	+	0	ID=BBa_K199008;Name=BBa_K199008
BBa_K199057	.	sequence_feature	139	149	.	+	0	ID=annotation2006984;Name=5' Context;Parent=BBa_K199008
BBa_K199057	.	sequence_feature	182	191	.	+	0	ID=annotation2006985;Name=Anticodon Loop;Parent=BBa_K199008
BBa_K199057	.	sequence_feature	243	282	.	+	0	ID=annotation2006986;Name=3' Context;Parent=BBa_K199008
BBa_K199057	.	ribosome_entry_site	499	510	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K199057	.	conserved_region	503	506	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199057	.	promoter	291	490	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K199057	.	non_covalent_binding_site	379	416	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K199057	.	non_covalent_binding_site	456	490	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K199057	.	promoter	427	432	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K199057	.	engineered_region	291	490	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K199057	.	promoter	451	456	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K199057	.	start_codon	463	463	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K199057	.	CDS	291	378	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
>BBa_K199057
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagggatccaattcggagagatg
ccggagcggctgaacggaccggttttgatggagaccggagtaggggcaactctaccgggggttcaaatccccctctctc
cgccactgcatatccttagcgaaagctaaggattttttttaagcttactagagcaatacgcaaaccgcctctccccgcg
cgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatg
tgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgg
ataacaatttcacacatactagagaaagaggagaaatactagatgccatcgcttcctccgaagacgttatcaaagagtt
catgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccg
tacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgc
agttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaagg
tttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggt
gagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggtt
gggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaaga
cggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaacc
gacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcact
ccaccggtgcttaataacgctgatagtgctagtgtagatcgc