##gff-version 3
##sequence-region BBa_K199060 1 1226
BBa_K199060	.	ribosome_entry_site	498	509	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K199060	.	conserved_region	502	505	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199060	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453
BBa_K199060	.	promoter	290	489	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K199060	.	non_covalent_binding_site	378	415	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K199060	.	promoter	450	455	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K199060	.	engineered_region	290	489	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K199060	.	CDS	290	377	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K199060	.	non_covalent_binding_site	455	489	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K199060	.	promoter	426	431	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K199060	.	start_codon	462	462	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K199060	.	engineered_region	516	1226	.	+	0	ID=BBa_K199034;Name=BBa_K199034
BBa_K199060	.	sequence_feature	1202	1226	.	+	0	ID=annotation2013329;Name=25 bp addition;Parent=BBa_K199034
BBa_K199060	.	sequence_feature	1196	1201	.	+	0	ID=annotation2013330;Name=Double Stop;Parent=BBa_K199034
BBa_K199060	.	sequence_feature	939	944	.	+	0	ID=annotation2011262;Name=Nco1 restriction site;Parent=BBa_K199034
BBa_K199060	.	sequence_feature	519	524	.	+	0	ID=annotation2011261;Name=5 base pair addition;Parent=BBa_K199034
BBa_K199060	.	mature_transcript_region	139	281	.	+	0	ID=BBa_K199028;Name=BBa_K199028
BBa_K199060	.	sequence_feature	182	190	.	+	0	ID=annotation2010430;Name=anticodon loop;Parent=BBa_K199028
BBa_K199060	.	sequence_feature	242	281	.	+	0	ID=annotation2010432;Name=3;Parent=BBa_K199028
BBa_K199060	.	sequence_feature	139	149	.	+	0	ID=annotation2010431;Name=5' context;Parent=BBa_K199028
>BBa_K199060
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagggatccaattcggagagatg
ccggagcggctgaacggaccggtttgaccgacaccggagtaggggcaactctaccgggggttcaaatccccctctctcc
gccactgcatatccttagcgaaagctaaggattttttttaagcttactagagcaatacgcaaaccgcctctccccgcgc
gttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgt
gagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgga
taacaatttcacacatactagagaaagaggagaaatactagatgcggtcgcttcctccgaagacgttatcaaagagttc
atgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgt
acgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgca
gttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggt
ttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtg
agttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttg
ggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagac
ggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccg
acatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactc
caccggtgcttaataacgctgatagtgctagtgtagatcgc