LOCUS       BBa_K199061             1226 bp    DNA     linear       22-Nov-2024
DEFINITION  CCCUC tRNA with RFP Reporter
ACCESSION   BBa_K199061
VERSION     BBa_K199061.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    182..190
                     /label=Anti-codon Loop
     misc_feature    139..149
                     /label=5' Context
     misc_feature    242..281
                     /label=3' Context
     misc_feature    139..281
                     /label=BBa_K199000
     CDS             290..377
                     /label=end of LacI coding region (inactive)
     misc_feature    290..489
                     /label=BBa_R0010
     misc_feature    378..415
                     /label=CAP binding site
     promoter        426..431
                     /label=-35
     promoter        450..455
                     /label=-10
     misc_feature    455..489
                     /label=LacI binding site
     misc_feature    462..462
                     /label=start
     promoter        290..489
                     /label=BBa_R0010
     misc_feature    502..505
                     /label=conserved
     RBS             498..509
                     /label=BBa_B0034
     misc_feature    519..523
                     /label=CCCUC 5 Base Addition
     misc_feature    939..944
                     /label=Nco1 restriction site
     misc_feature    1202..1226
                     /label=25 bp addition
     misc_feature    1196..1201
                     /label=Double Stop
     misc_feature    516..1226
                     /label=BBa_K199011
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagaggg atccaattcg gagagatgcc ggagcggctg aacggaccgg
      181 tctgagggtc accggagtag gggcaactct accgggggtt caaatccccc tctctccgcc
      241 actgcatatc cttagcgaaa gctaaggatt ttttttaagc ttactagagc aatacgcaaa
      301 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
      361 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
      421 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
      481 tttcacacat actagagaaa gaggagaaat actagatgcc ctcgcttcct ccgaagacgt
      541 tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt
      601 cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa
      661 agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta
      721 cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt
      781 cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt
      841 tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac
      901 caacttcccg tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac
      961 cgaacgtatg tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact
     1021 gaaagacggt ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt
     1081 tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga
     1141 ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata
     1201 acgctgatag tgctagtgta gatcgc
//