LOCUS       BBa_K199062             2223 bp    DNA     linear       25-Nov-2024
DEFINITION  pBad-RBS-RFP-pBad-RBS-Tet
ACCESSION   BBa_K199062
VERSION     BBa_K199062.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     RBS             139..153
                     /label=RBS-1\Strong
     RBS             146..150
                     /label=RBS
     misc_feature    139..153
                     /label=BBa_B0030
     RBS             139..153
                     /label=BBa_B0030
     promoter        1..153
                     /label=BBa_J3102
     CDS             160..834
                     /label=mrfp1
     misc_feature    841..865
                     /label=Help:Barcodes
     CDS             160..865
                     /label=BBa_E1010
     misc_feature    1..865
                     /label=BBa_K199030
     promoter        874..1003
                     /label=BBa_I13453
     RBS             1012..1026
                     /label=RBS-1\Strong
     RBS             1019..1023
                     /label=RBS
     misc_feature    1012..1026
                     /label=BBa_B0030
     RBS             1012..1026
                     /label=BBa_B0030
     CDS             1033..2223
                     /label=tetA(C) forward
     primer_bind     1033..1056
                     /label=Fwd primer J31007
     primer_bind     2204..2223
                     /label=Rev primer J31007
     misc_feature    2221..2223
                     /label=stop
     CDS             1033..2223
                     /label=BBa_J31007
     misc_feature    874..2223
                     /label=BBa_K199029
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagat taaagaggag aaatactaga tggcttcctc cgaagacgtt
      181 atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc
      241 gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa
      301 gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac
      361 ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc
      421 ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt
      481 acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc
      541 aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc
      601 gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg
      661 aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt
      721 cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac
      781 tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa
      841 cgctgatagt gctagtgtag atcgctacta gagacattga ttatttgcac ggcgtcacac
      901 tttgctatgc catagcattt ttatccataa gattagcgga tcctacctga cgctttttat
      961 cgcaactctc tactgtttct ccataccgtt tttttgggct agctactaga gattaaagag
     1021 gagaaatact agatgaaatc taacaatgcg ctcatcgtca tcctcggcac cgtcaccctg
     1081 gatgctgtag gcataggctt ggttatgccg gtactgccgg gcctcttgcg ggatatcgtc
     1141 cattccgaca gcatcgccag tcactatggc gtgctgctag cgctatatgc gttgatgcaa
     1201 tttctatgcg cacccgttct cggagcactg tccgaccgct ttggccgccg cccagtcctg
     1261 ctcgcttcgc tacttggagc cactatcgac tacgcgatca tggcgaccac acccgtcctg
     1321 tggatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
     1381 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
     1441 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc
     1501 atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg
     1561 ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaccgatgcc cttgagagcc
     1621 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg
     1681 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc
     1741 ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga
     1801 atcttgcacg ccctcgctca agccttcgtc actggccccg ccaccaaacg tttcggcgag
     1861 aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc
     1921 gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg
     1981 atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt
     2041 caaggatcgc tcgcggctct taccagccta acttcgatca ttggaccgct gatcgtcacg
     2101 gcgatttatg ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc
     2161 ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc
     2221 taa
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