##gff-version 3
##sequence-region BBa_K199062 1 2223
BBa_K199062	.	engineered_region	1	865	.	+	0	ID=BBa_K199030;Name=BBa_K199030
BBa_K199062	.	CDS	160	865	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K199030
BBa_K199062	.	engineered_tag	841	865	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K199062	.	CDS	160	834	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K199062	.	promoter	1	153	.	+	0	ID=BBa_J3102;Name=BBa_J3102;Parent=BBa_K199030
BBa_K199062	.	ribosome_entry_site	139	153	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_J3102
BBa_K199062	.	engineered_region	139	153	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K199062	.	ribosome_entry_site	146	150	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K199062	.	ribosome_entry_site	139	153	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K199062	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_J3102
BBa_K199062	.	engineered_region	874	2223	.	+	0	ID=BBa_K199029;Name=BBa_K199029
BBa_K199062	.	CDS	1033	2223	.	+	0	ID=BBa_J31007;Name=BBa_J31007;Parent=BBa_K199029
BBa_K199062	.	CDS	1033	2223	.	+	0	ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007
BBa_K199062	.	stop_codon	2221	2223	.	+	0	ID=annotation1910573;Name=stop;Parent=BBa_J31007
BBa_K199062	.	primer_binding_site	1033	1056	.	+	0	ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007
BBa_K199062	.	primer_binding_site	2204	2223	.	+	0	ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007
BBa_K199062	.	ribosome_entry_site	1012	1026	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K199029
BBa_K199062	.	engineered_region	1012	1026	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K199062	.	ribosome_entry_site	1019	1023	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K199062	.	ribosome_entry_site	1012	1026	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K199062	.	promoter	874	1003	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K199029
>BBa_K199062
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagattaaagaggagaaatacta
gatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgag
ttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtc
cgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacat
cccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgtt
accgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccg
acggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaa
aggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaa
aaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcg
ttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctact
agagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacct
gacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagattaaagaggagaaat
actagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttat
gccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgcta
tatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcg
cttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcat
cgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggct
cgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgcca
tctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcagga
gtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatg
actatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattt
tcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgc
tcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcg
ctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggca
tcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgc
ggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaac
gggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggcca
cctcgacctaa