Reporters RFP and Tet resistance (TetA), each controlled by medium-weak promoter pBad. Causes low expression of each reporter, can be mildly induced with L-arabinose.
Note that this part in a drug resistance plasmid subjects the cell to two drug resistances, including tetracycline. This may tend to make cells less hardy and grow slower.
Expresses tetracyline resistance [TetA(C) inner-membrane-associated protein] in the forward orientation.
This part is in plasmid pSB1A2, ampicillin resistance.
Expresses red fluorescent protein in the forward orientation. Induced by L-arabinose.
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
Campbell et al., PNAS v99 p7877 URL
monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
RBS.1 (strong) -- modified from R. Weiss
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]
This part is the coding region of the TetR gene. It requires a promoter and RBS for the cell to be able to express tetracyline resistance.
These parts are from the Resgistry 2006.
This part contains the promoter BBA_I13453 and the RBS BBA_B0030. There is an XbaI-SpeI mixed site between the two parts.
We neede to consider how to liagate the parts so that it did not contain restricttion sites between the parts.
Conversion of the iGEM parts registry to SBOL2.1