BBa_K199062
type: ComponentDefinition
persistent identity: BBa K199062
display id: BBa_K199062
version: 1
was generated by: iGEM to SBOL conversion
description: pBad-RBS-RFP-pBad-RBS-Tet
created: 2009-08-05T11:00:00Z
modified: 2015-05-08T01:11:19Z
mutable provenance: From the BioBrick Parts Registry.
owned by: james
owned by: myers
mutable description: Reporters RFP and Tet resistance (TetA), each controlled by medium-weak promoter pBad. Causes low expression of each reporter, can be mildly induced with L-arabinose.
discontinued: false
dominant: false
group u list: _295_
m user id: 0
owner id: 5112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Note that this part in a drug resistance plasmid subjects the cell to two drug resistances, including tetracycline. This may tend to make cells less hardy and grow slower.
star: false
creator: Olivia Ho-Shing
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K199029
component: BBa_K199030
sequence annotation: BBa_K199030
sequence annotation: BBa_K199029
sequence: https://synbiohub.org/public/igem/BBa_K199062_sequence/1

BBa_K199029
type: Component
persistent identity: component2262182
display id: component2262182
version: 1
top level: BBa_K199062
definition: BBa_K199029
access: public

BBa_K199030
type: Component
persistent identity: component2262172
display id: component2262172
version: 1
top level: BBa_K199062
definition: BBa_K199030
access: public

BBa_K199030
type: SequenceAnnotation
persistent identity: annotation2262172
display id: annotation2262172
version: 1
top level: BBa_K199062
location: https://synbiohub.org/public/igem/BBa_K199062/annotation2262172/range2262172/1
component: BBa_K199030

https://synbiohub.org/public/igem/BBa_K199062/annotation2262172/range2262172/1
type: Range
persistent identity: range2262172
display id: range2262172
version: 1
top level: BBa_K199062
direction: direction/reverse
start: 1
end: 865
orientation: inline

BBa_K199029
type: SequenceAnnotation
persistent identity: annotation2262182
display id: annotation2262182
version: 1
top level: BBa_K199062
location: https://synbiohub.org/public/igem/BBa_K199062/annotation2262182/range2262182/1
component: BBa_K199029

https://synbiohub.org/public/igem/BBa_K199062/annotation2262182/range2262182/1
type: Range
persistent identity: range2262182
display id: range2262182
version: 1
top level: BBa_K199062
direction: direction/reverse
start: 874
end: 2223
orientation: inline

BBa_K199029
type: ComponentDefinition
persistent identity: BBa K199029
display id: BBa_K199029
version: 1
was generated by: iGEM to SBOL conversion
description: pBad-RBS-Tet
created: 2009-07-10T11:00:00Z
modified: 2015-05-08T01:11:18Z
mutable provenance: From the BioBrick Parts Registry.
owned by: james
owned by: myers
mutable description: Expresses tetracyline resistance [TetA(C) inner-membrane-associated protein] in the forward orientation.
discontinued: false
dominant: false
experience: experience/Works
group u list: _295_
m user id: 0
owner id: 5112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part is in plasmid pSB1A2, ampicillin resistance.
star: false
creator: Olivia Ho-Shing
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0030
component: BBa_I13453
component: BBa_J31007
sequence annotation: BBa_I13453
sequence annotation: BBa_B0030
sequence annotation: BBa_J31007
sequence: https://synbiohub.org/public/igem/BBa_K199029_sequence/1

BBa_B0030
type: Component
persistent identity: component2011184
display id: component2011184
version: 1
top level: BBa_K199029
definition: BBa_B0030
access: public

BBa_I13453
type: Component
persistent identity: component2011182
display id: component2011182
version: 1
top level: BBa_K199029
definition: BBa_I13453
access: public

BBa_J31007
type: Component
persistent identity: component2011190
display id: component2011190
version: 1
top level: BBa_K199029
definition: tetA(C)f
access: public

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2011182
display id: annotation2011182
version: 1
top level: BBa_K199029
location: https://synbiohub.org/public/igem/BBa_K199029/annotation2011182/range2011182/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K199029/annotation2011182/range2011182/1
type: Range
persistent identity: range2011182
display id: range2011182
version: 1
top level: BBa_K199029
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2011184
display id: annotation2011184
version: 1
top level: BBa_K199029
location: https://synbiohub.org/public/igem/BBa_K199029/annotation2011184/range2011184/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K199029/annotation2011184/range2011184/1
type: Range
persistent identity: range2011184
display id: range2011184
version: 1
top level: BBa_K199029
direction: direction/forward
start: 139
end: 153
orientation: inline

BBa_J31007
type: SequenceAnnotation
persistent identity: annotation2011190
display id: annotation2011190
version: 1
top level: BBa_K199029
location: https://synbiohub.org/public/igem/BBa_K199029/annotation2011190/range2011190/1
component: BBa_J31007

https://synbiohub.org/public/igem/BBa_K199029/annotation2011190/range2011190/1
type: Range
persistent identity: range2011190
display id: range2011190
version: 1
top level: BBa_K199029
direction: direction/reverse
start: 160
end: 1350
orientation: inline

BBa_K199030
type: ComponentDefinition
persistent identity: BBa K199030
display id: BBa_K199030
version: 1
was generated by: iGEM to SBOL conversion
description: pBad-RBS-RFP
created: 2009-07-10T11:00:00Z
modified: 2015-05-08T01:11:18Z
mutable provenance: From the BioBricks Parts Registry.
owned by: james
owned by: myers
mutable description: Expresses red fluorescent protein in the forward orientation. Induced by L-arabinose.
discontinued: false
dominant: false
experience: experience/Works
group u list: _295_
m user id: 0
owner id: 5112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part is in plasmid pSB1A2, ampicillin resistance.
star: false
creator: Olivia Ho-Shing
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_J3102
component: BBa_E1010
sequence annotation: BBa_J3102
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K199030_sequence/1

BBa_J3102
type: Component
persistent identity: component2246380
display id: component2246380
version: 1
top level: BBa_K199030
definition: BBa_J3102
access: public

BBa_E1010
type: Component
persistent identity: component2246383
display id: component2246383
version: 1
top level: BBa_K199030
definition: mRFP1
access: public

BBa_J3102
type: SequenceAnnotation
persistent identity: annotation2246380
display id: annotation2246380
version: 1
top level: BBa_K199030
location: https://synbiohub.org/public/igem/BBa_K199030/annotation2246380/range2246380/1
component: BBa_J3102

https://synbiohub.org/public/igem/BBa_K199030/annotation2246380/range2246380/1
type: Range
persistent identity: range2246380
display id: range2246380
version: 1
top level: BBa_K199030
direction: direction/reverse
start: 1
end: 153
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2246383
display id: annotation2246383
version: 1
top level: BBa_K199030
location: https://synbiohub.org/public/igem/BBa_K199030/annotation2246383/range2246383/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K199030/annotation2246383/range2246383/1
type: Range
persistent identity: range2246383
display id: range2246383
version: 1
top level: BBa_K199030
direction: direction/reverse
start: 160
end: 865
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

tetA(C)f
type: ComponentDefinition
persistent identity: BBa J31007
display id: BBa_J31007
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: PsB1AT3
owned by: james
owned by: myers
mutable description: This part is the coding region of the TetR gene. It requires a promoter and RBS for the cell to be able to express tetracyline resistance.
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: It was cloned into PsB1A2 plamsid.
star: true
creator: Sabriya Rosemond, Erin Zwack
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tetA(C) forward
sequence annotation: Fwd primer J31007
sequence annotation: Rev primer J31007
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_J31007_sequence/1

tetA(C) forward
type: SequenceAnnotation
persistent identity: annotation1885000
display id: annotation1885000
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1885000/range1885000/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J31007/annotation1885000/range1885000/1
type: Range
persistent identity: range1885000
display id: range1885000
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1
end: 1191
orientation: inline

Fwd primer J31007
type: SequenceAnnotation
persistent identity: annotation1910571
display id: annotation1910571
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910571/range1910571/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31007/annotation1910571/range1910571/1
type: Range
persistent identity: range1910571
display id: range1910571
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1
end: 24
orientation: inline

Rev primer J31007
type: SequenceAnnotation
persistent identity: annotation1910572
display id: annotation1910572
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910572/range1910572/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31007/annotation1910572/range1910572/1
type: Range
persistent identity: range1910572
display id: range1910572
version: 1
top level: tetA(C)f
direction: direction/reverse
start: 1172
end: 1191
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1910573
display id: annotation1910573
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910573/range1910573/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J31007/annotation1910573/range1910573/1
type: Range
persistent identity: range1910573
display id: range1910573
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1189
end: 1191
orientation: inline

BBa_J3102
type: ComponentDefinition
persistent identity: BBa J3102
display id: BBa_J3102
version: 1
was generated by: iGEM to SBOL conversion
description: pBad : RBS
created: 2006-07-20T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: These parts are from the Resgistry 2006.
owned by: james
owned by: myers
mutable description: This part contains the promoter BBA_I13453 and the RBS BBA_B0030. There is an XbaI-SpeI mixed site between the two parts.
discontinued: false
dominant: true
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: We neede to consider how to liagate the parts so that it did not contain restricttion sites between the parts.
star: false
creator: Davidson and Missouri Western
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_I13453
component: BBa_B0030
sequence annotation: BBa_I13453
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_J3102_sequence/1

BBa_I13453
type: Component
persistent identity: component1886263
display id: component1886263
version: 1
top level: BBa_J3102
definition: BBa_I13453
access: public

BBa_B0030
type: Component
persistent identity: component1886265
display id: component1886265
version: 1
top level: BBa_J3102
definition: BBa_B0030
access: public

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1886263
display id: annotation1886263
version: 1
top level: BBa_J3102
location: https://synbiohub.org/public/igem/BBa_J3102/annotation1886263/range1886263/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_J3102/annotation1886263/range1886263/1
type: Range
persistent identity: range1886263
display id: range1886263
version: 1
top level: BBa_J3102
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1886265
display id: annotation1886265
version: 1
top level: BBa_J3102
location: https://synbiohub.org/public/igem/BBa_J3102/annotation1886265/range1886265/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J3102/annotation1886265/range1886265/1
type: Range
persistent identity: range1886265
display id: range1886265
version: 1
top level: BBa_J3102
direction: direction/forward
start: 139
end: 153
orientation: inline

https://synbiohub.org/public/igem/BBa_K199062_sequence/1
type: Sequence
persistent identity: BBa K199062 sequence
display id: BBa_K199062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagattaaagaggagaaatactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199029_sequence/1
type: Sequence
persistent identity: BBa K199029 sequence
display id: BBa_K199029_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagattaaagaggagaaatactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199030_sequence/1
type: Sequence
persistent identity: BBa K199030 sequence
display id: BBa_K199030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31007_sequence/1
type: Sequence
persistent identity: BBa J31007 sequence
display id: BBa_J31007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J3102_sequence/1
type: Sequence
persistent identity: BBa J3102 sequence
display id: BBa_J3102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagattaaagaggagaaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z