LOCUS       BBa_K199063             2082 bp    DNA     linear       29-Nov-2024
DEFINITION  pBad-RBS-RFP-RBS-Tet
ACCESSION   BBa_K199063
VERSION     BBa_K199063.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     RBS             139..153
                     /label=RBS-1\Strong
     RBS             146..150
                     /label=RBS
     misc_feature    139..153
                     /label=BBa_B0030
     RBS             139..153
                     /label=BBa_B0030
     promoter        1..153
                     /label=BBa_J3102
     CDS             160..834
                     /label=mrfp1
     misc_feature    841..865
                     /label=Help:Barcodes
     CDS             160..865
                     /label=BBa_E1010
     misc_feature    1..865
                     /label=BBa_K199030
     misc_feature    878..881
                     /label=conserved
     RBS             874..885
                     /label=BBa_B0034
     CDS             892..2082
                     /label=tetA(C) forward
     primer_bind     892..915
                     /label=Fwd primer J31007
     primer_bind     2063..2082
                     /label=Rev primer J31007
     misc_feature    2080..2082
                     /label=stop
     CDS             892..2082
                     /label=BBa_J31007
     misc_feature    874..2082
                     /label=BBa_S03710
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagat taaagaggag aaatactaga tggcttcctc cgaagacgtt
      181 atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc
      241 gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa
      301 gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac
      361 ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc
      421 ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt
      481 acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc
      541 aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc
      601 gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg
      661 aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt
      721 cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac
      781 tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa
      841 cgctgatagt gctagtgtag atcgctacta gagaaagagg agaaatacta gatgaaatct
      901 aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg
      961 gttatgccgg tactgccggg cctcttgcgg gatatcgtcc attccgacag catcgccagt
     1021 cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc acccgttctc
     1081 ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct acttggagcc
     1141 actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggatcctcta cgccggacgc
     1201 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     1261 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     1321 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     1381 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     1441 tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc
     1501 cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
     1561 ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg
     1621 agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa
     1681 gccttcgtca ctggccccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc
     1741 atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc
     1801 ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg
     1861 ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt
     1921 accagcctaa cttcgatcat tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg
     1981 agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt ctgcctcccc
     2041 gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct aa
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