BBa_K199063
type: ComponentDefinition
persistent identity: BBa K199063
display id: BBa_K199063
version: 1
was generated by: iGEM to SBOL conversion
description: pBad-RBS-RFP-RBS-Tet
created: 2009-08-05T11:00:00Z
modified: 2015-05-08T01:11:19Z
mutable provenance: From the BioBrick Parts Registry.
owned by: james
owned by: myers
mutable description: Reporters RFP and Tet resistance (TetA), under the singular control of the medium-weak promoter pBad. Designed to cause low expression of each reporter, can be mildly induced with L-arabinose.
discontinued: false
dominant: false
group u list: _295_
m user id: 0
owner id: 5112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The purpose of this composite part was to test if multiple genes can be expressed under a single promoter.
star: false
creator: Olivia Ho-Shing
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K199030
component: BBa_S03710
sequence annotation: BBa_K199030
sequence annotation: BBa_S03710
sequence: https://synbiohub.org/public/igem/BBa_K199063_sequence/1

BBa_K199030
type: Component
persistent identity: component2255421
display id: component2255421
version: 1
top level: BBa_K199063
definition: BBa_K199030
access: public

BBa_S03710
type: Component
persistent identity: component2255429
display id: component2255429
version: 1
top level: BBa_K199063
definition: BBa_S03710
access: public

BBa_K199030
type: SequenceAnnotation
persistent identity: annotation2255421
display id: annotation2255421
version: 1
top level: BBa_K199063
location: https://synbiohub.org/public/igem/BBa_K199063/annotation2255421/range2255421/1
component: BBa_K199030

https://synbiohub.org/public/igem/BBa_K199063/annotation2255421/range2255421/1
type: Range
persistent identity: range2255421
display id: range2255421
version: 1
top level: BBa_K199063
direction: direction/reverse
start: 1
end: 865
orientation: inline

BBa_S03710
type: SequenceAnnotation
persistent identity: annotation2255429
display id: annotation2255429
version: 1
top level: BBa_K199063
location: https://synbiohub.org/public/igem/BBa_K199063/annotation2255429/range2255429/1
component: BBa_S03710

https://synbiohub.org/public/igem/BBa_K199063/annotation2255429/range2255429/1
type: Range
persistent identity: range2255429
display id: range2255429
version: 1
top level: BBa_K199063
direction: direction/reverse
start: 874
end: 2082
orientation: inline

BBa_K199030
type: ComponentDefinition
persistent identity: BBa K199030
display id: BBa_K199030
version: 1
was generated by: iGEM to SBOL conversion
description: pBad-RBS-RFP
created: 2009-07-10T11:00:00Z
modified: 2015-05-08T01:11:18Z
mutable provenance: From the BioBricks Parts Registry.
owned by: james
owned by: myers
mutable description: Expresses red fluorescent protein in the forward orientation. Induced by L-arabinose.
discontinued: false
dominant: false
experience: experience/Works
group u list: _295_
m user id: 0
owner id: 5112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part is in plasmid pSB1A2, ampicillin resistance.
star: false
creator: Olivia Ho-Shing
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_J3102
component: BBa_E1010
sequence annotation: BBa_J3102
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K199030_sequence/1

BBa_J3102
type: Component
persistent identity: component2246380
display id: component2246380
version: 1
top level: BBa_K199030
definition: BBa_J3102
access: public

BBa_E1010
type: Component
persistent identity: component2246383
display id: component2246383
version: 1
top level: BBa_K199030
definition: mRFP1
access: public

BBa_J3102
type: SequenceAnnotation
persistent identity: annotation2246380
display id: annotation2246380
version: 1
top level: BBa_K199030
location: https://synbiohub.org/public/igem/BBa_K199030/annotation2246380/range2246380/1
component: BBa_J3102

https://synbiohub.org/public/igem/BBa_K199030/annotation2246380/range2246380/1
type: Range
persistent identity: range2246380
display id: range2246380
version: 1
top level: BBa_K199030
direction: direction/reverse
start: 1
end: 153
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2246383
display id: annotation2246383
version: 1
top level: BBa_K199030
location: https://synbiohub.org/public/igem/BBa_K199030/annotation2246383/range2246383/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K199030/annotation2246383/range2246383/1
type: Range
persistent identity: range2246383
display id: range2246383
version: 1
top level: BBa_K199030
direction: direction/reverse
start: 160
end: 865
orientation: inline

BBa_S03710
type: ComponentDefinition
persistent identity: BBa S03710
display id: BBa_S03710
version: 1
was generated by: iGEM to SBOL conversion
description: RBS-Tet
created: 2007-06-18T11:00:00Z
modified: 2015-05-08T01:14:29Z
owned by: james
owned by: myers
discontinued: false
dominant: true
experience: experience/None
group u list: _120_
m user id: 0
owner id: 1803
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Tom Crowley
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_J31007
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_J31007
sequence: https://synbiohub.org/public/igem/BBa_S03710_sequence/1

BBa_J31007
type: Component
persistent identity: component1934936
display id: component1934936
version: 1
top level: BBa_S03710
definition: tetA(C)f
access: public

BBa_B0034
type: Component
persistent identity: component1934931
display id: component1934931
version: 1
top level: BBa_S03710
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1934931
display id: annotation1934931
version: 1
top level: BBa_S03710
location: https://synbiohub.org/public/igem/BBa_S03710/annotation1934931/range1934931/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03710/annotation1934931/range1934931/1
type: Range
persistent identity: range1934931
display id: range1934931
version: 1
top level: BBa_S03710
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_J31007
type: SequenceAnnotation
persistent identity: annotation1934936
display id: annotation1934936
version: 1
top level: BBa_S03710
location: https://synbiohub.org/public/igem/BBa_S03710/annotation1934936/range1934936/1
component: BBa_J31007

https://synbiohub.org/public/igem/BBa_S03710/annotation1934936/range1934936/1
type: Range
persistent identity: range1934936
display id: range1934936
version: 1
top level: BBa_S03710
direction: direction/reverse
start: 19
end: 1209
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

tetA(C)f
type: ComponentDefinition
persistent identity: BBa J31007
display id: BBa_J31007
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: PsB1AT3
owned by: james
owned by: myers
mutable description: This part is the coding region of the TetR gene. It requires a promoter and RBS for the cell to be able to express tetracyline resistance.
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: It was cloned into PsB1A2 plamsid.
star: true
creator: Sabriya Rosemond, Erin Zwack
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tetA(C) forward
sequence annotation: Fwd primer J31007
sequence annotation: Rev primer J31007
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_J31007_sequence/1

tetA(C) forward
type: SequenceAnnotation
persistent identity: annotation1885000
display id: annotation1885000
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1885000/range1885000/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J31007/annotation1885000/range1885000/1
type: Range
persistent identity: range1885000
display id: range1885000
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1
end: 1191
orientation: inline

Fwd primer J31007
type: SequenceAnnotation
persistent identity: annotation1910571
display id: annotation1910571
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910571/range1910571/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_J31007/annotation1910571/range1910571/1
type: Range
persistent identity: range1910571
display id: range1910571
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1
end: 24
orientation: inline

Rev primer J31007
type: SequenceAnnotation
persistent identity: annotation1910572
display id: annotation1910572
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910572/range1910572/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_J31007/annotation1910572/range1910572/1
type: Range
persistent identity: range1910572
display id: range1910572
version: 1
top level: tetA(C)f
direction: direction/reverse
start: 1172
end: 1191
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1910573
display id: annotation1910573
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910573/range1910573/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J31007/annotation1910573/range1910573/1
type: Range
persistent identity: range1910573
display id: range1910573
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1189
end: 1191
orientation: inline

BBa_J3102
type: ComponentDefinition
persistent identity: BBa J3102
display id: BBa_J3102
version: 1
was generated by: iGEM to SBOL conversion
description: pBad : RBS
created: 2006-07-20T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: These parts are from the Resgistry 2006.
owned by: james
owned by: myers
mutable description: This part contains the promoter BBA_I13453 and the RBS BBA_B0030. There is an XbaI-SpeI mixed site between the two parts.
discontinued: false
dominant: true
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: We neede to consider how to liagate the parts so that it did not contain restricttion sites between the parts.
star: false
creator: Davidson and Missouri Western
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_I13453
component: BBa_B0030
sequence annotation: BBa_I13453
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_J3102_sequence/1

BBa_I13453
type: Component
persistent identity: component1886263
display id: component1886263
version: 1
top level: BBa_J3102
definition: BBa_I13453
access: public

BBa_B0030
type: Component
persistent identity: component1886265
display id: component1886265
version: 1
top level: BBa_J3102
definition: BBa_B0030
access: public

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1886263
display id: annotation1886263
version: 1
top level: BBa_J3102
location: https://synbiohub.org/public/igem/BBa_J3102/annotation1886263/range1886263/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_J3102/annotation1886263/range1886263/1
type: Range
persistent identity: range1886263
display id: range1886263
version: 1
top level: BBa_J3102
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1886265
display id: annotation1886265
version: 1
top level: BBa_J3102
location: https://synbiohub.org/public/igem/BBa_J3102/annotation1886265/range1886265/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J3102/annotation1886265/range1886265/1
type: Range
persistent identity: range1886265
display id: range1886265
version: 1
top level: BBa_J3102
direction: direction/forward
start: 139
end: 153
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: BBa_B0030
sequence annotation: RBS-1\Strong
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

https://synbiohub.org/public/igem/BBa_K199063_sequence/1
type: Sequence
persistent identity: BBa K199063 sequence
display id: BBa_K199063_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199030_sequence/1
type: Sequence
persistent identity: BBa K199030 sequence
display id: BBa_K199030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03710_sequence/1
type: Sequence
persistent identity: BBa S03710 sequence
display id: BBa_S03710_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31007_sequence/1
type: Sequence
persistent identity: BBa J31007 sequence
display id: BBa_J31007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J3102_sequence/1
type: Sequence
persistent identity: BBa J3102 sequence
display id: BBa_J3102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagattaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z