LOCUS       BBa_K199069             1620 bp    DNA     linear       22-Dec-2024
DEFINITION  RFP-RBS-ShpOmpC-pLux_rev-RBS-GFP
ACCESSION   BBa_K199069
VERSION     BBa_K199069.1  
FEATURES             Location/Qualifiers
     CDS             1..678
                     /label=mRFP reverse
     primer_bind     659..678
                     /label=Spe mRFP F
     primer_bind     1..20
                     /label=Xba mRFP R
     misc_feature    1..678
                     /label=BBa_J31008
     RBS             687..701
                     /label=Reverse RBS
     RBS             687..701
                     /label=BBa_J44001
     promoter        710..808
                     /label=BBa_K199017
     promoter        817..871
                     /label=BBa_K199052
     RBS             880..894
                     /label=RBS-1\Strong
     RBS             887..891
                     /label=RBS
     misc_feature    880..894
                     /label=BBa_B0030
     RBS             880..894
                     /label=BBa_B0030
     CDS             901..1620
                     /label=GFP protein
     CDS             901..1620
                     /label=BBa_E0040
ORIGIN
        1 ttaagcaccg gtggagtgac gaccttcagc acgttcgtac tgttcaacga tggtgtagtc
       61 ttcgttgtgg gaggtgatgt ccagtttgat gtcggttttg taagcacccg gcagctgaac
      121 cggtttttta gccatgtagg tggttttaac ttcagcgtcg tagtgaccac cgtctttcag
      181 tttcagacgc attttgattt cacctttcag agcaccgtct tccgggtaca tacgttcggt
      241 ggaagcttcc caacccatgg tttttttctg cataaccgga ccgtcggacg ggaagttggt
      301 accacgcagt ttaactttgt agatgaactc accgtcttgc agggaggagt cctgggtaac
      361 ggtaacaaca ccaccgtctt cgaagttcat aacacgttcc catttgaaac cttccgggaa
      421 ggacagtttc aggtagtccg ggatgtcagc cgggtgttta acgtaagctt tggaaccgta
      481 ctggaactgc ggggacagga tgtcccaagc gaacggcagc ggaccacctt tggtaacttt
      541 cagtttagcg gtctgggtac cttcgtacgg acgaccttca ccttcacctt cgatttcgaa
      601 ctcgtgaccg ttaacggaac cttccatacg aactttgaaa cgcatgaact ctttgataac
      661 gtcttcggag gaagccatta ctagagtttc tcctctttaa ttactagagt ttacattttg
      721 aaacatctat agcgataaat gaaacatctt aaaagtttta gtatcatatt cgtgttggat
      781 tattctgcat ttttggggag aatggactta ctagagttta ttcgactata acaaaccatt
      841 ttcttgcgta aacctgtacg atcctacagg ttactagaga ttaaagagga gaaatactag
      901 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      961 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
     1021 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
     1081 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
     1141 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
     1201 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
     1261 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
     1321 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
     1381 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
     1441 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     1501 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
     1561 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataataa
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