BBa_K199076
1
BBa_K199076
RBS RFP with CUACU Addition
2009-09-19T11:00:00Z
2015-05-08T01:11:19Z
The template of the gene comes from Part BBa_E1010. We designed a forward primer that included RBS,ATG, the 5-bp addition CTACT and the first 20 nucleotides on the 5' end of RFP after ATG. This made the edited 5' sequence for our RFP.
This RBS RFP reporter gene is a modification of BBa_E1010 with BBa_B0034; it has a 5-bp addition, CUACU, after the start codon and before the rest of the gene. This is used in conjunction with the CUACU tRNA(Part K199046). These CUACU tRNAs suppress the frameshift caused by this 5-bp addition in the reporter.
There is also a 25 bp addition after the double stop of the gene and before the Bio Brick Suffix. This addition is presumed to not effect the translation of the protein.
Sequence and Features
false
false
_295_
0
5118
9
It's complicated
false
None
false
Clifton Davis
annotation2023124
1
NcoI Restriction Site
range2023124
1
442
442
annotation2023126
1
25-bp addition
range2023126
1
705
729
annotation2023125
1
5-bp CUACU Addition
range2023125
1
705
729
BBa_K199076_sequence
1
aaagaggagaaatactagatgctactgcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z